Human IFN-β enzyme-linked immunoassay kit
Catalog# EH0017
Human IFN-β enzyme-linked immunoassay kit
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ELISA
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Human
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15.63~1000 pg/ml
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unconjugated
Overview
Product Name
Human IFN-β enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.35 pg/ml (50 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0349 | 0.0318 | 0.0334 | |
| 15.63 | 0.0798 | 0.0747 | 0.0773 | 0.0439 |
| 31.25 | 0.1220 | 0.1323 | 0.1272 | 0.0938 |
| 62.50 | 0.2001 | 0.1991 | 0.1996 | 0.1663 |
| 125.00 | 0.3930 | 0.4183 | 0.4057 | 0.3723 |
| 250.00 | 0.8126 | 0.7841 | 0.7984 | 0.7650 |
| 500.00 | 1.7670 | 1.6420 | 1.7045 | 1.6712 |
| 1000.00 | 3.5520 | 3.5080 | 3.5300 | 3.4967 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 20.0 | 99.1 | 304.4 | 21.3 | 101.4 | 317.2 |
| Standard Deviation | 1.2 | 5.8 | 14.3 | 1.5 | 4.2 | 15.7 |
| Coefficient of Variation (%) | 6.2 | 5.9 | 4.7 | 7.1 | 4.1 | 5.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IFN beta into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 102% to 129% with an overall mean recovery of 121%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-2.08 | 53 | 1.03 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IFN-β in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Application in Publications
| ELISA | See 2 publications below |
Application in Publications
| Human | See 2 publications below |
Target
UNIPROT
Alias
Function
Citation
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Development and Comprehensive Evaluation of Scarless Circularization Systems for Circular RNA Therapeutics
Journal: Molecular Therapy-Nucleic Acids
DOI: 10.1016/j.omtn.2025.102587
IF: 6.1
Application: ELISA
Reactivity: Human
Publish date: 2025 Jun
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The cGAS-STING pathway activates transcription factor TFEB to stimulate lysosome biogenesis and pathogen clearance
Journal: Immunity
DOI:
IF: 25.5
Application: ELISA
Reactivity: Human
Publish date: 2024 Dec