Human IgA enzyme-linked immunoassay kit(one step)
Catalog# EHY0335
Human IgA enzyme-linked immunoassay kit(one step)
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ELISA
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Human
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1.37 ~1000 ng/ml
Overview
Product Name
Human IgA enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.01ng/ml (50 μl)；1.10ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0050 | 0.0052 | 0.0051 | |
| 1.37 | 0.0105 | 0.0105 | 0.0105 | 0.0054 |
| 4.12 | 0.0246 | 0.0251 | 0.0249 | 0.0198 |
| 12.35 | 0.0731 | 0.0733 | 0.0732 | 0.0681 |
| 37.04 | 0.2061 | 0.2088 | 0.2075 | 0.2024 |
| 111.11 | 0.6025 | 0.6268 | 0.6147 | 0.6096 |
| 333.33 | 1.6850 | 1.7020 | 1.6935 | 1.6884 |
| 1000.00 | 3.3010 | 3.2490 | 3.2750 | 3.2699 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 12.7 | 75.0 | 277.9 | 13.0 | 76.1 | 293.6 |
| Standard Deviation | 0.6 | 2.7 | 14.9 | 0.4 | 4.3 | 17.2 |
| Coefficient of Variation (%) | 4.6 | 3.6 | 5.4 | 3.4 | 5.6 | 5.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Human IgA into health Human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 109% with an overall mean recovery of 101%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (mg/ml) | Detectable (%) | Mean of Detectable (mg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1.13-8.76 | 100 | 4.86 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Human IgA in this assay. No medical histories were available for the donors.n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
1
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
Alias
Hepatocellular carcinoma-associated protein TB6 antibody
Ig alpha 1 chain C region antibody
Ig alpha 2 chain C region antibody
IGHA antibody
IGHA1 antibody
IGHA2 antibody
Immunoglobulin heavy constant alpha 1 antibody
Immunoglobulin heavy constant alpha 2 A2m marker antibody
Immunoglobulin heavy constant alpha 2 antibody
PIgR antibody
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