Human IL-6R enzyme-linked immunoassay kit(one step)
Catalog# EHY0207
Human IL-6R enzyme-linked immunoassay kit(one step)
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
0.41~100 ng/ml
Overview
Product Name
Human IL-6R enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.05 ng/ml (50 μl)；0.56 ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0079 | 0.0086 | 0.0083 | |
| 0.41 | 0.0145 | 0.0145 | 0.0145 | 0.0063 |
| 1.02 | 0.0287 | 0.0276 | 0.0282 | 0.0199 |
| 2.56 | 0.0740 | 0.0729 | 0.0735 | 0.0652 |
| 6.40 | 0.2382 | 0.2381 | 0.2382 | 0.2299 |
| 16.00 | 0.8391 | 0.8159 | 0.8275 | 0.8193 |
| 40.00 | 2.2770 | 2.1820 | 2.2295 | 2.2213 |
| 100.00 | 3.8800 | 3.9150 | 3.8975 | 3.8893 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 1.8 | 8.5 | 29.5 | 1.8 | 8.6 | 30.6 |
| Standard Deviation | 0.0 | 0.2 | 1.1 | 0.0 | 0.2 | 0.7 |
| Coefficient of Variation (%) | 2.2 | 2.2 | 3.7 | 2.4 | 2.3 | 2.4 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 IL-6R，未加人 IL-6R 的血清作为本底，计算回收率。回收率的范围从 83 % 至 116 %，平均回收率为 95%。
Sample Value
| Sample Matrix | Evaluated Sample | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 16.73-65.67 | 100 | 36.52 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
60min
Standard Curve Gradient
7点
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
CD 126
CD126
CD126 antigen
gp80
IL 6R 1
IL 6R alpha
IL 6R
IL-6 receptor alpha chain
IL-6 receptor subunit alpha
IL-6R 1
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