Human IL-6 Super Sensitivity enzyme-linked immunoassay kit

Usd: 619 Special Discount

Specification

96T

Bulk Order Quote | Customization Request

Catalog# EHSS0001

Human IL-6 Super Sensitivity enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

0.14~100 pg/ml

Overview

Product Name

Human IL-6 Super Sensitivity enzyme-linked immunoassay kit

Product Type

Elisa Kit

Sensitivity

0.11 pg/ml (50 μl)；0.33 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0565	0.0573	0.0569
0.14	0.0621	0.0635	0.0628	0.0059
0.41	0.0701	0.0717	0.0709	0.0140
1.23	0.0919	0.0878	0.0899	0.0330
3.70	0.1406	0.1335	0.1371	0.0802
11.11	0.3042	0.2996	0.3019	0.2450
33.33	0.7676	0.7670	0.7673	0.7104
100.00	2.0951	1.9914	2.0433	1.9864

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	1.6	10.1	31.0	1.7	9.9	29.9
Standard Deviation	0.1	0.3	0.8	0.1	0.4	1.1
Coefficient of Variation (%)	6.8	2.7	2.5	6.7	3.8	3.6

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human IL-6 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 84% to 114% with an overall mean recovery of 99%.

Sample Value

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	2.45-53.36	100	23.94

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-6 in this assay. No medical histories were available for the donors.

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

120min

Standard Curve Gradient

7 Points/3 Folds

Incubation Count

Detectable Sample

Dosing Volume

50 μl/10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Target

UNIPROT

SwissProt: P05231 Human

Alias

Interleukin BSF 2

B cell differentiation factor

B cell stimulatory factor 2

B-cell stimulatory factor 2

BSF 2

BSF-2

BSF2

CDF

CTL differentiation factor

Hepatocyte stimulatory factor

Expand

Function

Interleukin 6 (IL-6) is a pleiotropic, a-helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature human IL-6 is 183 amino acids (aa) in length and shares 39% aa sequence identity with mouse and rat IL-6. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. Cells known to express IL-6 include CD8+ T cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells (under the influence of endothelins), sympathetic neurons, cerebral cortex neurons, adrenal medulla chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cells, fetal and adult astrocytes, neutrophils, monocytes, eosinophils, colonic epithelial cells, B1 B cells and pancreatic islet beta cells. IL-6 production is generally correlated with cell activation and is normally kept in control by glucocorticoids, catecholamines, and secondary sex steroids. Normal human circulating IL-6 is in the 1 pg/mL range, with slight elevations during the menstrual cycle, modest elevations in certain cancers, and large elevations after surgery. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 Ra, triggering IL-6 Ra association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 Ra are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 Ra elicit responses from gp130-expressing cells that lack cell surface IL-6 Ra. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 Ra is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 Ra but not from other cytokines that use gp130 as a co-receptor. IL-6, along with TNF-a and IL-1, drives the acute inflammatory response. IL-6 is almost solely responsible for fever and the acute phase response in the liver, and it is important in the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in conditions such as obesity, insulin resistance, inflammatory bowel disease, arthritis, and sepsis. IL-6 modulates bone resorption and is a major effector of inflammatory joint destruction in rheumatoid arthritis through its promotion of Th17 cell development and activity. It contributes to atherosclerotic plaque development and destabilization as well as the development of inflammation-associated carcinogenesis.

Human IL-6 Super Sensitivity enzyme-linked immunoassay kit

Usd: 619 Special Discount

Specification

Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Detectable Sample

Dosing Volume

Product Features

Storage Condition

Target

UNIPROT

Alias

Function

Same Target and Pathway Products

Human IFN-β enzyme-linked immunoassay kit

Human ULBP1/NKG2DL1 enzyme-linked immunoassay kit

Human CD163 enzyme-linked immunoassay kit

Human Renin enzyme-linked immunoassay kit

Human/Mouse MASP2 enzyme-linked immunoassay kit

Human IL-6 Super Sensitivity enzyme-linked immunoassay kit

Human/Monkey Complement C3 enzyme-linked immunoassay kit(one step)

Lentivirus HIV-1 p24 enzyme-linked immunoassay kit（one step）

Human CD273/B7-DC/PD-L2 enzyme-linked immunoassay kit（one step）

Human Complement factor B enzyme-linked immunoassay kit(one step)

Human SNAP25 enzyme-linked immunoassay kit

Human CX3CL1/Fractalking enzyme-linked immunoassay kit

Human CXCL2/MIP2/GRO beta enzyme-linked immunoassay kit

Human tPA enzyme-linked immunoassay kit(one step)

Human IL-3 enzyme-linked immunoassay kit

Human Myeloperoxidase(MPO) enzyme-linked immunoassay kit(one step)

Human B7-H6/NCR3LG1 enzyme-linked immunoassay kit

Human CD48 enzyme-linked immunoassay kit

Human MMP-7 enzyme-linked immunoassay kit

Human CTLA-4 enzyme-linked immunoassay kit

Human/Monkey/Canine CCL5/RANTES enzyme-linked immunoassay kit(one step)

Human Lipoprotein A/LPA enzyme-linked immunoassay kit(one step)

Human CTGF/CCN2 enzyme-linked immunoassay kit(one step)

Human Proprotein Convertase 9/PCSK9 enzyme-linked immunoassay kit（one step）

Human CCL2/MCP-1 enzyme-linked immunoassay kit

Human CCL5/RANTES enzyme-linked immunoassay kit

Human FGF-19 enzyme-linked immunoassay kit

Human Antithrombin III/AT III enzyme-linked immunoassay kit(one step)

Human CD73 enzyme-linked immunoassay kit

Human CD47 enzyme-linked immunoassay kit

Human Neuropilin-1/NRP1 enzyme-linked immunoassay kit（one step）

Human IL-17E/IL-25 enzyme-linked immunoassay kit

Human/Monkey/Canine CXCL10/IP-10 enzyme-linked immunoassay kit

Human/Monkey/Canine Osteopontin（OPN） enzyme-linked immunoassay kit

Human Total HSP60 enzyme-linked immunoassay kit

Human IL-13R alpha 2 enzyme-linked immunoassay kit

Human CHIT1 enzyme-linked immunoassay kit

Human RBP4 enzyme-linked immunoassay kit（one step）

Human Caspase-9 enzyme-linked immunoassay kit

Human SAA1 enzyme-linked immunoassay kit

Human/Monkey Albumin enzyme-linked immunoassay kit(one step)

Human/Monkey PCSK9 enzyme-linked immunoassay kit (one step)

Human TGF-α enzyme-linked immunoassay kit

Human Leptin enzyme-linked immunoassay kit

Human CD27/TNFRSF7 enzyme-linked immunoassay kit

Human MERTK enzyme-linked immunoassay kit（one step）

Human ICAM-1 / CD54 enzyme-linked immunoassay kit

Human/Monkey/Canine IL-18 enzyme-linked immunoassay kit

Human Endoglin/CD105 enzyme-linked immunoassay kit

Human IL-1RA/IL-1F3 enzyme-linked immunoassay kit

Human beta-NGF enzyme-linked immunoassay kit

Human IL-15 enzyme-linked immunoassay kit

Human PAI-1/Serpin E1 enzyme-linked immunoassay kit(one step)

Human CD28 enzyme-linked immunoassay kit

Human CCL25 enzyme-linked immunoassay kit