Human IL-6 Super Sensitivity enzyme-linked immunoassay kit
Catalog# EHSS0001
Human IL-6 Super Sensitivity enzyme-linked immunoassay kit
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ELISA
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Human
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0.14~100 pg/ml
Overview
Product Name
Human IL-6 Super Sensitivity enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.11 pg/ml (50 μl)；0.33 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0565 | 0.0573 | 0.0569 | |
| 0.14 | 0.0621 | 0.0635 | 0.0628 | 0.0059 |
| 0.41 | 0.0701 | 0.0717 | 0.0709 | 0.0140 |
| 1.23 | 0.0919 | 0.0878 | 0.0899 | 0.0330 |
| 3.70 | 0.1406 | 0.1335 | 0.1371 | 0.0802 |
| 11.11 | 0.3042 | 0.2996 | 0.3019 | 0.2450 |
| 33.33 | 0.7676 | 0.7670 | 0.7673 | 0.7104 |
| 100.00 | 2.0951 | 1.9914 | 2.0433 | 1.9864 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 1.6 | 10.1 | 31.0 | 1.7 | 9.9 | 29.9 |
| Standard Deviation | 0.1 | 0.3 | 0.8 | 0.1 | 0.4 | 1.1 |
| Coefficient of Variation (%) | 6.8 | 2.7 | 2.5 | 6.7 | 3.8 | 3.6 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人IL-6，未加人 IL-6 的血清作为本底，计算回收率。回收率的范围从 84 % 至 114 %，平均回收率为 99 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 2.45-53.36 | 100 | 23.94 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Interleukin BSF 2
B cell differentiation factor
B cell stimulatory factor 2
B-cell stimulatory factor 2
BSF 2
BSF-2
BSF2
CDF
CTL differentiation factor
Hepatocyte stimulatory factor
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