Human LIGHT/TNFSF 14 enzyme-linked immunoassay kit
Catalog# EH0356
Human LIGHT/TNFSF 14 enzyme-linked immunoassay kit
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sELISA
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Human
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1.37 ~1000 pg/ml
Overview
Product Name
Human LIGHT/TNFSF 14 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.02pg/ml (50 μl)； 1.79pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0056 | 0.0054 | 0.0055 | |
| 1.37 | 0.0182 | 0.0157 | 0.0170 | 0.0115 |
| 4.12 | 0.0454 | 0.0464 | 0.0459 | 0.0404 |
| 12.35 | 0.1327 | 0.1317 | 0.1322 | 0.1267 |
| 37.04 | 0.3915 | 0.3874 | 0.3895 | 0.3840 |
| 111.11 | 1.0448 | 1.1238 | 1.0843 | 1.0788 |
| 333.33 | 2.6482 | 2.7299 | 2.6891 | 2.6836 |
| 1000.00 | 4.4024 | 4.5220 | 4.4622 | 4.4567 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 18.2 | 99.3 | 351.5 | 18.2 | 92.8 | 322.2 |
| Standard Deviation | 0.6 | 2.5 | 18.2 | 1.1 | 5.8 | 19.2 |
| Coefficient of Variation (%) | 3.3 | 2.5 | 5.2 | 6.2 | 6.3 | 5.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human LIGHT/TNFSF14 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 99% with an overall mean recovery of 94%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1.30-7.01 | 100 | 3.49 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human LIGHT/TNFSF14 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
CD 258
CD258
CD258 antigen
Delta transmembrane LIGHT
Herpes virus entry mediator ligand
Herpesvirus entry mediator A
Herpesvirus entry mediator ligand
herpesvirus entry mediator-ligand
HVEM L
HVEM-L
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