Human IGFBP1 enzyme-linked immunoassay kit(one step)
Catalog# EHY0258
Human IGFBP1 enzyme-linked immunoassay kit(one step)
-
ELISA
-
Human
-
13.72~10000 pg/ml
Overview
Product Name
Human IGFBP1 enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
1.13 pg/ml (50 μl)；15.75 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0057 | 0.0053 | 0.0055 | |
| 13.72 | 0.0083 | 0.0082 | 0.0083 | 0.0028 |
| 41.15 | 0.0165 | 0.0154 | 0.0160 | 0.0105 |
| 123.46 | 0.0426 | 0.0463 | 0.0445 | 0.0390 |
| 370.37 | 0.1283 | 0.1181 | 0.1232 | 0.1177 |
| 1111.11 | 0.3838 | 0.3516 | 0.3677 | 0.3622 |
| 3333.33 | 1.0460 | 0.9522 | 0.9991 | 0.9936 |
| 10000.00 | 2.3570 | 2.2190 | 2.2880 | 2.2825 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 236.9 | 1109.1 | 3258.2 | 230.4 | 1101.7 | 3259.2 |
| Standard Deviation | 16.1 | 73.4 | 254.8 | 4.6 | 64.8 | 84.5 |
| Coefficient of Variation (%) | 6.8 | 6.5 | 7.8 | 2.8 | 3.0 | 3.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Human IGFBP1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 119% with an overall mean recovery of 105%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 0.72-26.08 | 100 | 8.56 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Human IGFBP1 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃