Human NKp46/NCR1 enzyme-linked immunoassay kit
Catalog# EH0221
Human NKp46/NCR1 enzyme-linked immunoassay kit
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sELISA
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Human
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1.37~1000 pg/ml
Overview
Product Name
Human NKp46/NCR1 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.06 pg/ml (50 μl)；1.09 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0119 | 0.0135 | 0.0127 | |
| 1.37 | 0.0232 | 0.0240 | 0.0236 | 0.0232 |
| 4.12 | 0.0501 | 0.0495 | 0.0498 | 0.0501 |
| 12.35 | 0.1274 | 0.1232 | 0.1253 | 0.1274 |
| 37.04 | 0.3634 | 0.3362 | 0.3498 | 0.3634 |
| 111.11 | 0.9253 | 0.9344 | 0.9298 | 0.9253 |
| 333.33 | 2.2430 | 2.0810 | 2.1620 | 2.2430 |
| 1000.00 | 3.7100 | 3.7730 | 3.7415 | 3.7100 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 30.2 | 162.4 | 401.4 | 22.2 | 134.6 | 462.6 |
| Standard Deviation | 1.1 | 4.7 | 6.9 | 1.3 | 4.8 | 13.7 |
| Coefficient of Variation (%) | 3.7 | 2.9 | 1.7 | 5.6 | 3.5 | 3.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested Twenty-two times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human NKp46/NCR1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 86% to 109% with an overall mean recovery of 95%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| serum | 30 | 115.20-331.30 | 100 | 218.98 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of NKp46/NCR1 in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Activating NK receptor NK p46
CD335
FLJ99094
hNKp46
Ly94
Lymphocyte antigen 94
Lymphocyte antigen 94 homolog (activating NK receptor
NK p46)
Lymphocyte antigen 94 homolog
Natural cytotoxicity triggering receptor 1
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