Human Total HSP60 enzyme-linked immunoassay kit
Catalog# EH0060
Human Total HSP60 enzyme-linked immunoassay kit
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ELISA
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Human
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4.10~1000 pg/ml
Overview
Product Name
Human Total HSP60 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
3.22 pg/ml (50 μl)；4.45 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0310 | 0.0327 | 0.0319 | |
| 4.10 | 0.0436 | 0.0462 | 0.0449 | 0.0131 |
| 10.24 | 0.0678 | 0.0691 | 0.0685 | 0.0366 |
| 25.60 | 0.1196 | 0.1236 | 0.1216 | 0.0898 |
| 64.00 | 0.2535 | 0.2621 | 0.2578 | 0.2260 |
| 160.00 | 0.5522 | 0.5783 | 0.5653 | 0.5334 |
| 400.00 | 1.2460 | 1.2700 | 1.2580 | 1.2262 |
| 1000.00 | 2.5840 | 2.5540 | 2.5690 | 2.5372 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 26.6 | 99.0 | 277.0 | 27.8 | 101.2 | 284.9 |
| Standard Deviation | 2.3 | 5.6 | 21.7 | 1.8 | 5.3 | 14.5 |
| Coefficient of Variation (%) | 8.5 | 5.6 | 7.8 | 6.3 | 5.2 | 5.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Total HSP60 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 66% to 73% with an overall mean recovery of 69%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-20.90 | 50 | 8.98 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-12/IL-23 p40 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
60 kDa chaperonin
60 kDa heat shock protein, mitochondrial
CH60_HUMAN
Chaperonin 60
Chaperonin, 60-KD
CPN60
fa04a05
GROEL
heat shock 60kDa protein 1 (chaperonin)
Heat shock protein 1 (chaperonin)
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