Human TIM4 enzyme-linked immunoassay kit(one step)
Catalog# EHY0194
Human TIM4 enzyme-linked immunoassay kit(one step)
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ELISA
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Human
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0.14~100 ng/ml
Overview
Product Name
Human TIM4 enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.001 ng/ml (50 μl)；0.03 ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0066 | 0.0066 | 0.0066 | |
| 0.14 | 0.0584 | 0.0587 | 0.0586 | 0.0520 |
| 0.41 | 0.1645 | 0.1618 | 0.1632 | 0.1566 |
| 1.23 | 0.4574 | 0.4633 | 0.4604 | 0.4538 |
| 3.70 | 1.2320 | 1.2350 | 1.2335 | 1.2269 |
| 11.11 | 2.6720 | 2.6900 | 2.6810 | 2.6744 |
| 33.33 | 3.4720 | 3.4610 | 3.4665 | 3.4599 |
| 100.00 | 3.7210 | 3.6020 | 3.6615 | 3.6549 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 1.7 | 12.8 | 30.6 | 1.6 | 13.8 | 31.6 |
| Standard Deviation | 0.1 | 1.1 | 4.1 | 0.1 | 1.5 | 5.2 |
| Coefficient of Variation (%) | 3.1 | 4.5 | 3.2 | 2.7 | 3.9 | 4.2 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 TIM4，未加人 TIM4 的血清作为本底，计算回收率。回收率的范围从 89 % 至 113 %，平均回收率为 98%。
Sample Value
| Sample Matrix | Evaluated Sample | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 4.94-10.86 | 100 | 7.18 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
SMUCKLER
Spleen
mucin-containing
knockout of lymphotoxin protein
T cell immunoglobulin and mucin domain containing protein 4
T-cell immunoglobulin and mucin domain containing 4
T-cell immunoglobulin and mucin domain containing molecule
T-cell immunoglobulin and mucin domain-containing protein 4
T-cell immunoglobulin and mucin domains-containing protein 4
T-cell membrane protein 4
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