Human CD115/M-CSF R/CSF-1-R enzyme-linked immunoassay kit（one step）
Catalog# EHY0168
Human CD115/M-CSF R/CSF-1-R enzyme-linked immunoassay kit（one step）
-
ELISA
-
Human
-
0.41~300 ng/ml
Overview
Product Name
Human CD115/M-CSF R/CSF-1-R enzyme-linked immunoassay kit（one step）
Product Type
Elisa Kit
Sensitivity
0.01 ng/ml (50 μl)；0.08 ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0056 | 0.0054 | 0.0055 | |
| 0.41 | 0.0188 | 0.0196 | 0.0192 | 0.0137 |
| 1.23 | 0.0493 | 0.0501 | 0.0497 | 0.0442 |
| 3.70 | 0.1445 | 0.1453 | 0.1449 | 0.1394 |
| 11.11 | 0.4360 | 0.4285 | 0.4323 | 0.4268 |
| 33.33 | 1.2330 | 1.2430 | 1.2380 | 1.2325 |
| 100.00 | 3.2110 | 3.1820 | 3.1965 | 3.1910 |
| 300.00 | 4.2906 | 4.2706 | 4.2806 | 4.2751 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 7.6 | 38.8 | 148.5 | 6.5 | 33.6 | 102.7 |
| Standard Deviation | 0.3 | 1.8 | 2.7 | 0.4 | 2.0 | 2.5 |
| Coefficient of Variation (%) | 3.6 | 4.5 | 1.8 | 6.0 | 6.0 | 2.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CD115/M-CSF R/CSF-1-R into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 99% with an overall mean recovery of 89%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 174.98-741.02 | 100 | 502.94 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD115/M-CSF R/CSF-1-R in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
C FMS
CD 115
CD115
CD115 antigen
CFMS
Colony stimulating factor 1 receptor
Colony stimulating factor I receptor
CSF 1 R
CSF 1R
CSF-1 receptor
Expand