Human/Mouse C-Fos enzyme-linked immunoassay kit

Usd: 499 Special Discount

Specification

96T

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Catalog# EH0304

Human/Mouse C-Fos enzyme-linked immunoassay kit

Application

sELISA

Reactivity

Human

Standard curve range

1.37 ~1000 pg/ml

Overview

Product Name

Human/Mouse C-Fos enzyme-linked immunoassay kit

Product Type

Elisa Kit

Sensitivity

0.42 pg/ml (50 μl)；0.92 pg/ml (10 μl)；

pg/ml	O.D.		Average	Corrected
0.00	0.0137	0.0147	0.0142
1.37	0.0205	0.0195	0.0200	0.0058
4.12	0.0329	0.0322	0.0326	0.0183
12.35	0.0713	0.0742	0.0728	0.0585
37.04	0.2012	0.1856	0.1934	0.1792
111.11	0.5080	0.5080	0.5080	0.4938
333.33	1.3790	1.3730	1.3760	1.3618
1000.00	3.4090	3.2950	3.3520	3.3378

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	24.1	112.9	337.4	23.8	115.5	326.8
Standard Deviation	0.7	3.8	8.7	0.3	4.0	11.7
Coefficient of Variation (%)	3.0	3.4	2.6	1.1	3.5	3.6

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human/mouse C-Fos into health human/mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 93% to 116% with an overall mean recovery of102%.

Sample Value

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Human serum	30	0.48-14.65	100	5.28
Mouse serum	30	0.19-121.65	100	9.33

Serum/Plasma – Thirty samples from apparently healthy volunteers and thirty samples from apparently healthy mice were evaluated for the presence of human/mouse C-Fos in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

120min

Standard Curve Gradient

7 Points/3 Folds

Incubation Count

Detectable Sample

Dosing Volume

50 μl/10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Target

UNIPROT

SwissProt: P01100 Human

SwissProt: P01101 Mouse

Alias

Activator protein 1

AP 1

C FOS

Cellular oncogene c fos

Cellular oncogene fos

FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS)

FBJ murine osteosarcoma viral oncogene homolog

FBJ murine osteosarcoma viral v fos oncogene homolog

FBJ Osteosarcoma Virus

FOS

Expand

Function

c-Fos is an intermediate early gene that is one of four members of the FOS family of activator protein-1 (AP-1) transcription factors, which also includes Fra-1, Fra-2, and FosB. Under the FOS gene, human c-Fos is synthesized as a protein of 308 amino acids (aa) with a basic leucine zipper (bZip) domain important for dimerization, and a basic domain for interacting with DNA, with a theoretical molecular weight of ~40.6 kDa. c-Fos can heterodimerize with members of the JUN family (c-Jun, JunB, and JunD) to form the active transcription factor AP-1 complex. AP-1 related proteins bind TPA-related responsive elements (TRE), cAMP responsive elements (CRE), and related sequences, with c-Fos:c-Jun dimers partial to TRE sites. In response to stimuli, c-Fos, which is encoded by protooncogenes, has a role in cell proliferation, differentiation, and transformation. A variety of stimuli can increase c-Fos expression such as growth factors, proinflammatory cytokines, UV radiation, neurotransmitters, hormones, injury, and stress. c-Fos has long been used as a marker for neuronal activity and is associated with neural and behavioral responses following stimuli. Mouse studies have revealed that c-Fos is important for efficient neurogenesis and cortical development. Additionally, c-Fos signal can be used as a molecular marker for learning and memory, such as recognition and fear. Studies have found that repeated positive stimuli result in increased Fos expression while, conversely, repeated negative value stimuli are indicated by decreased signal. Intermediate early genes have also been implicated in neuropsychiatric disorders including showing altered c-Fos expression in a schizophrenia animal model. Furthermore, antipsychotics and antidepressants are both capable of impacting c-Fos expression.

Human/Mouse C-Fos enzyme-linked immunoassay kit

Usd: 499 Special Discount

Specification

Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Detectable Sample

Dosing Volume

Product Features

Storage Condition

Target

UNIPROT

Alias

Function

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