Human von Willebrand Factor enzyme-linked immunoassay kit（one step）
Catalog# EHY0138
Human von Willebrand Factor enzyme-linked immunoassay kit（one step）
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ELISA
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Human
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4.69~300 ng/ml
Overview
Product Name
Human von Willebrand Factor enzyme-linked immunoassay kit（one step）
Product Type
Elisa Kit
Sensitivity
0.02 ng/ml (50 μl)；0.69 ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0064 | 0.0058 | 0.0061 | |
| 4.69 | 0.0354 | 0.0345 | 0.0350 | 0.0293 |
| 9.38 | 0.0859 | 0.0789 | 0.0824 | 0.0798 |
| 18.75 | 0.2116 | 0.1990 | 0.2053 | 0.2055 |
| 37.50 | 0.5367 | 0.5317 | 0.5342 | 0.5306 |
| 75.00 | 1.3890 | 1.3180 | 1.3535 | 1.3829 |
| 150.00 | 2.5750 | 2.7090 | 2.6420 | 2.5689 |
| 300.00 | 3.6260 | 3.6480 | 3.6370 | 3.6199 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 5.7 | 32.8 | 151.8 | 5.3 | 31.3 | 113.6 |
| Standard Deviation | 0.2 | 1.7 | 10.4 | 0.1 | 2.0 | 7.7 |
| Coefficient of Variation (%) | 2.7 | 5.2 | 6.8 | 2.6 | 6.4 | 6.7 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 von Willebrand Factor，未加人 von Willebrand Factor 的血清作为本底，计算回收率。回收率的范围从 94 % 至 126 %，平均回收率为 109 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 10.53-1936.87 | 100 | 1056.08 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Coagulation factor VIII
Coagulation factor VIII VWF
F8VWF
Factor VIII related antigen
von Willebrand antigen 2
von Willebrand antigen II
Von Willebrand disease
VWD
vWF
VWF_HUMAN