Human beta Amyloid 1-40 enzyme-linked immunoassay kit
Catalog# EH0111
Human beta Amyloid 1-40 enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
31.25~2000 pg/ml
Overview
Product Name
Human beta Amyloid 1-40 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
2.92 pg/ml (50 μl)；8.40 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0446 | 0.0451 | 0.0449 | |
| 31.25 | 0.0817 | 0.0770 | 0.0794 | 0.0345 |
| 62.50 | 0.1291 | 0.1349 | 0.1320 | 0.0871 |
| 125.00 | 0.2772 | 0.2812 | 0.2792 | 0.2343 |
| 250.00 | 0.5233 | 0.5129 | 0.5181 | 0.4732 |
| 500.00 | 1.0246 | 1.0388 | 1.0317 | 0.9868 |
| 1000.00 | 1.8213 | 1.8056 | 1.8135 | 1.7686 |
| 2000.00 | 3.3130 | 3.3240 | 3.3185 | 3.2736 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 757.9 | 168.5 | 48.0 | 703.8 | 202.7 | 42.7 |
| Standard Deviation | 40.9 | 7.1 | 1.8 | 22.1 | 5.6 | 2.4 |
| Coefficient of Variation (%) | 5.4 | 4.2 | 3.7 | 3.1 | 2.8 | 5.6 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 beta Amyloid 1-40，未加人 beta Amyloid 1-40 的血清作为本底，计算回收率。回收率的范围从 84 % 至 122 %，平均回收率为 105 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.- 907.22 | 37.5 | 198.85 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Function
β-Amyloid (1-40), Iowa mutation 是一种有生物活性的肽。(β淀粉样蛋白前体基因的几种突变会导致许多家族患常染色体显性阿尔茨海默病。爱荷华州突变（其中 Asp 23 被 Asn 取代）与严重的大脑淀粉样蛋白血管病 (CAA) 相关。受影响的个体在 APP 694 位上有一个错义突变。突变的 β-淀粉样肽聚集得更快，并形成有毒原纤维。)