Human beta Amyloid 1-40 enzyme-linked immunoassay kit
Catalog# EH0111
Human beta Amyloid 1-40 enzyme-linked immunoassay kit
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ELISA
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Human
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31.25~2000 pg/ml
Overview
Product Name
Human beta Amyloid 1-40 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
2.92 pg/ml (50 μl)；8.40 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0446 | 0.0451 | 0.0449 | |
| 31.25 | 0.0817 | 0.0770 | 0.0794 | 0.0345 |
| 62.50 | 0.1291 | 0.1349 | 0.1320 | 0.0871 |
| 125.00 | 0.2772 | 0.2812 | 0.2792 | 0.2343 |
| 250.00 | 0.5233 | 0.5129 | 0.5181 | 0.4732 |
| 500.00 | 1.0246 | 1.0388 | 1.0317 | 0.9868 |
| 1000.00 | 1.8213 | 1.8056 | 1.8135 | 1.7686 |
| 2000.00 | 3.3130 | 3.3240 | 3.3185 | 3.2736 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 757.9 | 168.5 | 48.0 | 703.8 | 202.7 | 42.7 |
| Standard Deviation | 40.9 | 7.1 | 1.8 | 22.1 | 5.6 | 2.4 |
| Coefficient of Variation (%) | 5.4 | 4.2 | 3.7 | 3.1 | 2.8 | 5.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human beta Amyloid 1-40 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 84% to 122% with an overall mean recovery of 105%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) | |
|---|---|---|---|---|---|
| Serum | 30 | n.d.- | 907.22 | 37.5 | 198.85 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of beta Amyloid 1-40 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃