Human VEGFR2/KDR enzyme-linked immunoassay kit
Catalog# EH0200
Human VEGFR2/KDR enzyme-linked immunoassay kit
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ELISA
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Human
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6.86~5000 pg/ml
Overview
Product Name
Human VEGFR2/KDR enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
1.54 pg/ml (50 μl)；9.53 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0221 | 0.0195 | 0.0208 | |
| 6.86 | 0.0336 | 0.0305 | 0.0321 | 0.0113 |
| 20.58 | 0.0500 | 0.0446 | 0.0473 | 0.0265 |
| 61.73 | 0.1072 | 0.1073 | 0.1073 | 0.0865 |
| 185.19 | 0.2788 | 0.2483 | 0.2636 | 0.2428 |
| 555.56 | 0.7927 | 0.7507 | 0.7717 | 0.7509 |
| 1666.67 | 2.1021 | 2.0794 | 2.0908 | 2.0700 |
| 5000.00 | 4.1378 | 4.1878 | 4.1628 | 4.1420 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 121.47 | 525.25 | 1597.38 | 116.40 | 515.89 | 1636.98 |
| Standard Deviation | 6.01 | 32.20 | 84.55 | 1.94 | 15.10 | 85.29 |
| Coefficient of Variation (%) | 4.9 | 6.1 | 5.3 | 1.7 | 2.9 | 5.2 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 VEGFR2/KDR，未加人 VEGFR2/KDR 的血清作为本底，计算回收率。回收率的范围从 99 % 至 110 %，平均回收率为 107 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 0.28-24.70 | 100 | 9.93 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
CD309
CD309 antigen
EC 2.7.10.1
Fetal liver kinase 1
FLK-1
FLK1
FLK1, mouse, homolog of
Kdr
Kinase insert domain receptor (a type III receptor tyrosine kinase)
Kinase insert domain receptor
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