Human tPA enzyme-linked immunoassay kit(one step)
Catalog# EHY0158
Human tPA enzyme-linked immunoassay kit(one step)
-
ELISA
-
Human
-
0.14~100 ng/ml
Overview
Product Name
Human tPA enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.03 ng/ml (50 μl)；0.10 ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0058 | 0.0064 | 0.0061 | |
| 0.14 | 0.0107 | 0.0096 | 0.0102 | 0.0041 |
| 0.41 | 0.0195 | 0.0181 | 0.0188 | 0.0127 |
| 1.23 | 0.0457 | 0.0450 | 0.0454 | 0.0393 |
| 3.70 | 0.1406 | 0.1261 | 0.1334 | 0.1273 |
| 11.11 | 0.4191 | 0.3776 | 0.3984 | 0.3923 |
| 33.33 | 1.1900 | 1.1700 | 1.1800 | 1.1739 |
| 100.00 | 2.8370 | 2.8780 | 2.8575 | 2.8514 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 1.8 | 9.0 | 29.2 | 1.8 | 9.4 | 31.7 |
| Standard Deviation | 0.1 | 0.4 | 1.5 | 0.1 | 0.4 | 1.8 |
| Coefficient of Variation (%) | 5.7 | 4.6 | 5.1 | 5.2 | 3.9 | 5.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human tPA into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 97% to 115% with an overall mean recovery of 104%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 3.24-20.65 | 100 | 9.25 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of tPA in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Alteplase
DKFZp686I03148
Plasminogen activator tissue
Plasminogen activator tissue type
PLAT
Reteplase
t PA
T Plasminogen Activator
t-PA
T-plasminogen activator
Expand