Human CD62L/L-selectin enzyme-linked immunoassay kit
Catalog# EH0188
Human CD62L/L-selectin enzyme-linked immunoassay kit
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ELISA
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Human
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6.86~5000 pg/ml
Overview
Product Name
Human CD62L/L-selectin enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
1.51 pg/ml (50 μl)；11.18 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0162 | 0.0160 | 0.0161 | |
| 6.86 | 0.0274 | 0.0251 | 0.0263 | 0.0102 |
| 20.58 | 0.0507 | 0.0456 | 0.0482 | 0.0321 |
| 61.73 | 0.1143 | 0.1132 | 0.1138 | 0.0977 |
| 185.19 | 0.2860 | 0.2738 | 0.2799 | 0.2638 |
| 555.56 | 0.8035 | 0.7703 | 0.7869 | 0.7708 |
| 1666.67 | 2.0140 | 1.8580 | 1.9360 | 1.9199 |
| 5000.00 | 3.9330 | 3.5250 | 3.7290 | 3.7129 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 138.9 | 668.2 | 2131.8 | 115.4 | 538.4 | 1870.3 |
| Standard Deviation | 9.3 | 46.1 | 138.8 | 9.0 | 32.0 | 144.1 |
| Coefficient of Variation (%) | 6.7 | 6.9 | 6.5 | 7.8 | 7.9 | 7.7 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 CD62L/L-selectin，未加人 CD62L/L-selectin 的血清作为本底，计算回收率。回收率的范围从 90 % 至 124 %，平均回收率为 98%。
Sample Value
| Sample Matrix | Evaluated Sample | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 23.83-794.23 | 100 | 427.33 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
A.11
AI528707
CD62 antigen ligand
CD62 antigen-like family member L
CD62L
gp90-MEL
IgA nephropathy, susceptibility to, included
L Selectin
L-selectin
LAM-1
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