Human DR3/TNFRSF 25 enzyme-linked immunoassay kit
Catalog# EH0316
Human DR3/TNFRSF 25 enzyme-linked immunoassay kit
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ELISA
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Human
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122.88 ~30000 pg/ml
Overview
Product Name
Human DR3/TNFRSF 25 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
25.35 pg/ml (50 μl)；102.54 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0332 | 0.0314 | 0.0323 | |
| 122.88 | 0.0509 | 0.0466 | 0.0488 | 0.0165 |
| 307.20 | 0.0743 | 0.0731 | 0.0737 | 0.0414 |
| 768.00 | 0.1352 | 0.1326 | 0.1339 | 0.1016 |
| 1920.00 | 0.2828 | 0.2852 | 0.2840 | 0.2517 |
| 4800.00 | 0.6329 | 0.6434 | 0.6382 | 0.6059 |
| 12000.00 | 1.5230 | 1.4900 | 1.5065 | 1.4742 |
| 30000.00 | 3.2040 | 3.0130 | 3.1085 | 3.0762 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 640.2 | 3195.0 | 9036.4 | 774.0 | 3811.5 | 10947.1 |
| Standard Deviation | 37.9 | 72.3 | 242.3 | 17.6 | 154.8 | 159.2 |
| Coefficient of Variation (%) | 2.3 | 5.9 | 2.7 | 2.3 | 4.1 | 1.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human DR3/TNFRSF25 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 101% to 120% with an overall mean recovery of 118%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1247.74-2549.10 | 100 | 1887.31 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human DR3/TNFRSF25 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃