Human CCL16/LEC enzyme-linked immunoassay kit(one step)
Catalog# EHY0294
Human CCL16/LEC enzyme-linked immunoassay kit(one step)
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ELISA
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Human
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0.78~50 ng/ml
Overview
Product Name
Human CCL16/LEC enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.01 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0054 | 0.0054 | 0.0054 | |
| 0.78 | 0.1235 | 0.1153 | 0.1194 | |
| 0.1140 | 1.56 | 0.2368 | 0.2407 | 0.2388 |
| 0.2334 | 3.13 | 0.4504 | 0.4531 | 0.4518 |
| 0.4464 | 6.25 | 0.8144 | 0.8275 | 0.8210 |
| 0.8156 | 12.50 | 1.4330 | 1.5100 | 1.4715 |
| 1.4661 | 25.00 | 2.4360 | 2.4490 | 2.4425 |
| 2.4371 | 50.00 | 3.5390 | 3.6880 | 3.6135 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 1.3 | 6.9 | 17.2 | 1.5 | 6.4 | 16.2 |
| Standard Deviation | 0.1 | 0.3 | 0.8 | 0.1 | 0.3 | 0.6 |
| Coefficient of Variation (%) | 4.3 | 4.8 | 4.5 | 4.1 | 4.7 | 3.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CCL16/LEC into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 109% with an overall mean recovery of 93%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 4.33-13.11 | 100 | 8.66 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human CCL16/LEC in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
1
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃