Human IL-17RA/CD217 enzyme-linked immunoassay kit
Catalog# EH0278
Human IL-17RA/CD217 enzyme-linked immunoassay kit
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ELISA
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Human
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78.13 ~5000 pg/ml
Overview
Product Name
Human IL-17RA/CD217 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
1.50 pg/ml (50 μl)；11.14 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0557 | 0.0552 | 0.0555 | |
| 78.13 | 0.1731 | 0.1738 | 0.1735 | 0.1180 |
| 156.25 | 0.2638 | 0.2971 | 0.2805 | 0.2250 |
| 312.50 | 0.4855 | 0.5111 | 0.4983 | 0.4429 |
| 625.00 | 0.8464 | 0.9013 | 0.8739 | 0.8184 |
| 1250.00 | 1.4570 | 1.4230 | 1.4400 | 1.3846 |
| 2500.00 | 2.1660 | 2.1390 | 2.1525 | 2.0971 |
| 5000.00 | 3.0440 | 3.1250 | 3.0845 | 3.0291 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 46.8 | 366.5 | 1128.6 | 47.2 | 358.4 | 1196.9 |
| Standard Deviation | 3.5 | 17.0 | 46.3 | 2.1 | 17.4 | 77.3 |
| Coefficient of Variation (%) | 7.6 | 4.6 | 4.1 | 4.4 | 4.8 | 6.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-17RA/CD217 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 83% to 107% with an overall mean recovery of 95%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 900.28-3172.52 | 94 | 1705.32 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human IL-17RA/CD217 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃