Human Fas Ligand/TNFSF6 enzyme-linked immunoassay kit
Catalog# EH0291
Human Fas Ligand/TNFSF6 enzyme-linked immunoassay kit
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ELISA
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Human
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1.37~1000 pg/ml
Overview
Product Name
Human Fas Ligand/TNFSF6 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.12 pg/ml (50 μl)；0.84 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0129 | 0.0122 | 0.0126 | |
| 1.37 | 0.0209 | 0.0206 | 0.0208 | 0.0082 |
| 4.12 | 0.0321 | 0.0325 | 0.0323 | 0.0198 |
| 12.35 | 0.0828 | 0.0862 | 0.0845 | 0.0720 |
| 37.04 | 0.2466 | 0.2461 | 0.2464 | 0.2338 |
| 111.11 | 0.7016 | 0.7212 | 0.7114 | 0.6989 |
| 333.33 | 1.8430 | 1.8680 | 1.8555 | 1.8430 |
| 1000.00 | 3.9250 | 4.0234 | 3.9742 | 3.9617 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 16.9 | 101.4 | 310.4 | 18.1 | 102.9 | 329.4 |
| Standard Deviation | 0.5 | 3.0 | 7.4 | 0.4 | 2.5 | 12.6 |
| Coefficient of Variation (%) | 2.8 | 2.9 | 2.4 | 2.4 | 2.4 | 3.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Fas Ligand/TNFSF6 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 96% to 127% with an overall mean recovery of 112%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 8.47-38.67 | 100 | 18.25 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human Fas Ligand/TNFSF6 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃