Human IL-3R alpha/CD123 enzyme-linked immunoassay kit
Catalog# EH0134
Human IL-3R alpha/CD123 enzyme-linked immunoassay kit
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ELISA
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Human
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156.25~10000 pg/ml
Overview
Product Name
Human IL-3R alpha/CD123 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
22.75 pg/ml (50 μl)；129.58 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0491 | 0.0475 | 0.0483 | |
| 156.25 | 0.0991 | 0.0987 | 0.0989 | 0.0506 |
| 312.50 | 0.1488 | 0.1489 | 0.1489 | 0.1006 |
| 625.00 | 0.2471 | 0.2517 | 0.2494 | 0.2011 |
| 1250.00 | 0.4688 | 0.4276 | 0.4482 | 0.3999 |
| 2500.00 | 0.8168 | 0.8312 | 0.8240 | 0.7757 |
| 5000.00 | 1.5140 | 1.5180 | 1.5160 | 1.4677 |
| 10000.00 | 2.7410 | 2.7560 | 2.7485 | 2.7002 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 219.2 | 1152.6 | 3499.9 | 194.9 | 1053.1 | 3264.2 |
| Standard Deviation | 12.1 | 67.3 | 170.5 | 12.8 | 58.3 | 145.9 |
| Coefficient of Variation (%) | 5.5 | 5.8 | 4.9 | 6.5 | 5.5 | 4.5 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 IL-3R alpha/CD123，未加人 IL-3R alpha/CD123 的血清作为本底，计算回收率。回收率的范围从 80 % 至 95 %，平均回收率为 86 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 804.66-2639.86 | 100 | 1600.42 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
CD123
CD123 antigen
hIL 3Ra
hIL-3Ra
hIL3Ra
IL 3 receptor alpha SP2 isoform
IL 3R alpha
IL-3 receptor subunit alpha
IL-3R subunit alpha
IL-3R-alpha
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