Human/Mouse/Rat FGF1 enzyme-linked immunoassay kit
Catalog# EH0302
Human/Mouse/Rat FGF1 enzyme-linked immunoassay kit
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sELISA
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Human
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1.37 ~1000 pg/ml
Overview
Product Name
Human/Mouse/Rat FGF1 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.21 pg/ml (50 μl)；0.98 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0171 | 0.0159 | 0.0165 | |
| 1.37 | 0.0238 | 0.0247 | 0.0243 | 0.0078 |
| 4.12 | 0.0405 | 0.0407 | 0.0406 | 0.0241 |
| 12.35 | 0.0853 | 0.0860 | 0.0857 | 0.0692 |
| 37.04 | 0.2135 | 0.2223 | 0.2179 | 0.2014 |
| 111.11 | 0.6743 | 0.6299 | 0.6521 | 0.6356 |
| 333.33 | 1.7250 | 1.5970 | 1.6610 | 1.6445 |
| 1000.00 | 3.6830 | 3.6440 | 3.6635 | 3.6470 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 18.8 | 96.3 | 320.4 | 22.3 | 117.8 | 344.7 |
| Standard Deviation | 0.6 | 5.4 | 16.5 | 0.8 | 4.1 | 11.7 |
| Coefficient of Variation (%) | 3.2 | 5.6 | 5.1 | 3.4 | 3.5 | 3.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human FGF1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 92% to 112% with an overall mean recovery of 102%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Human Serum | 30 | n.d.-5.34 | 6.25 | 0.33 |
| Rat Serum | 30 | n.d.-138.45 | 50 | 15.90 |
| Mouse Serum | 30 | n.d.-378.55 | 31.25 | 32.65 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human/rat/mouse FGF1 in this assay. No medical histories were available for the donors.n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
Alias
Acidic fibroblast growth factor
aFGF
Beta endothelial cell growth factor
Beta-endothelial cell growth factor
ECGF
ECGF beta
ECGF-beta
ECGFA
ECGFB
Endothelial Cell Growth Factor alpha
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