Human CD254/RANKL/TNFSF11 enzyme-linked immunoassay kit
Catalog# EH0214
Human CD254/RANKL/TNFSF11 enzyme-linked immunoassay kit
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ELISA
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Human
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6.86~5000 pg/ml
Overview
Product Name
Human CD254/RANKL/TNFSF11 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.20 pg/ml (50 μl)；0.76 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0113 | 0.0117 | 0.0115 | |
| 6.86 | 0.0282 | 0.0296 | 0.0289 | 0.0174 |
| 20.58 | 0.0583 | 0.0597 | 0.0590 | 0.0475 |
| 61.73 | 0.1615 | 0.1627 | 0.1621 | 0.1506 |
| 185.19 | 0.4068 | 0.4528 | 0.4298 | 0.4183 |
| 555.56 | 1.0560 | 1.1330 | 1.0945 | 1.0830 |
| 1666.67 | 2.2150 | 2.1870 | 2.2010 | 2.1895 |
| 5000.00 | 3.2400 | 3.2890 | 3.2645 | 3.2530 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 98.6 | 567.8 | 1800.9 | 81.2 | 446.1 | 1484.8 |
| Standard Deviation | 5.2 | 41.2 | 140.4 | 3.2 | 13.9 | 70.5 |
| Coefficient of Variation (%) | 5.3 | 7.3 | 7.8 | 3.9 | 3.1 | 4.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Human CD254/RANKL/TNFSF11 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 102% with an overall mean recovery of 91%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 0.63-9.14 | 100 | 4.14 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD254/RANKL/TNFSF11 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
CD254
hRANKL2
ODF
OPGL
OPTB2
Osteoclast differentiation factor
Osteoprotegerin ligand
Rank Ligand
RANKL
Receptor activator of nuclear factor kappa B ligand
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