Human Neuropeptide Y enzyme-linked immunoassay kit
Catalog# EH0292
Human Neuropeptide Y enzyme-linked immunoassay kit
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ELISA
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Human
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15.63~1000 pg/ml
Overview
Product Name
Human Neuropeptide Y enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
2.36 pg/ml (50 μl)；30.57 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0072 | 0.0065 | 0.0069 | |
| 15.63 | 0.0106 | 0.0097 | 0.0102 | 0.0033 |
| 31.25 | 0.0174 | 0.0166 | 0.0170 | 0.0102 |
| 62.50 | 0.0407 | 0.0405 | 0.0406 | 0.0338 |
| 125.00 | 0.1256 | 0.1201 | 0.1229 | 0.1160 |
| 250.00 | 0.3874 | 0.3546 | 0.3710 | 0.3642 |
| 500.00 | 1.1710 | 1.1270 | 1.1490 | 1.1422 |
| 1000.00 | 3.0160 | 2.9140 | 2.9650 | 2.9582 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 17.6 | 98.2 | 288.9 | 17.1 | 101.1 | 301.2 |
| Standard Deviation | 1.3 | 1.9 | 6.5 | 0.7 | 3.0 | 8.6 |
| Coefficient of Variation (%) | 7.3 | 1.9 | 2.2 | 4.2 | 3.0 | 2.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Neuropeptide Y into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 123% with an overall mean recovery of 103%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Human Serum | 30 | n.d.-23.20 | 12.5 | 18.05 |
Serum/Plasma– Thirty samples from apparently healthy volunteers/mouse serum were evaluated for the presence of human Neuropeptide Y in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃