Human Amphiregulin/ARGE enzyme-linked immunoassay kit
Catalog# EH0273
Human Amphiregulin/ARGE enzyme-linked immunoassay kit
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ELISA
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Human
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31.25 ~2000 pg/ml
Overview
Product Name
Human Amphiregulin/ARGE enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
8.05 pg/ml (50 μl)；49.15 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0254 | 0.0254 | 0.0254 | |
| 31.25 | 0.0404 | 0.0372 | 0.0388 | 0.0134 |
| 62.50 | 0.0582 | 0.0572 | 0.0577 | 0.0323 |
| 125.00 | 0.0945 | 0.0989 | 0.0967 | 0.0713 |
| 250.00 | 0.2107 | 0.2184 | 0.2146 | 0.1892 |
| 500.00 | 0.5350 | 0.5538 | 0.5444 | 0.5190 |
| 1000.00 | 1.5250 | 1.4390 | 1.4820 | 1.4566 |
| 2000.00 | 3.5120 | 3.5410 | 3.5265 | 3.5011 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 33.5 | 192.6 | 626.7 | 37.0 | 200.0 | 630.3 |
| Standard Deviation | 2.3 | 7.0 | 26.0 | 0.3 | 12.0 | 11.8 |
| Coefficient of Variation (%) | 6.7 | 3.6 | 4.1 | 0.7 | 6.0 | 1.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Amphiregulin/AREG into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 86% to 104% with an overall mean recovery of 90%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-1000.69 | 94 | 238.91 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human Amphiregulin/AREG in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃