Human CCL23/MPIF-1 enzyme-linked immunoassay kit
Catalog# EH0130
Human CCL23/MPIF-1 enzyme-linked immunoassay kit
-
ELISA
-
Human
-
4.1~1000 pg/ml
Overview
Product Name
Human CCL23/MPIF-1 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
1.10 pg/ml (50 μl)；1.66 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0095 | 0.0087 | 0.0091 | |
| 4.10 | 0.0230 | 0.0207 | 0.0219 | 0.0128 |
| 10.24 | 0.0500 | 0.0457 | 0.0479 | 0.0388 |
| 25.60 | 0.1250 | 0.1321 | 0.1286 | 0.1195 |
| 64.00 | 0.3903 | 0.3985 | 0.3944 | 0.3853 |
| 160.00 | 1.3330 | 1.2530 | 1.2930 | 1.2839 |
| 400.00 | 3.3930 | 3.7000 | 3.5465 | 3.5374 |
| 1000.00 | 4.3920 | 4.4231 | 4.4076 | 4.3985 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 19.9 | 99.0 | 302.5 | 21.6 | 107.5 | 319.6 |
| Standard Deviation | 1.2 | 3.9 | 9.7 | 1.2 | 4.1 | 13.2 |
| Coefficient of Variation (%) | 5.8 | 3.9 | 3.2 | 5.6 | 3.8 | 4.1 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 CCL23/MPIF-1，未加人 CCL23/MPIF-1 的血清作为本底，计算回收率。回收率的范围从 93 % 至 120 %，平均回收率为 107 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 131.30-479.56 | 100.0 | 371.04 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
C C motif chemokine ligand 23
C-C motif chemokine 23
C6 beta chemokine
CCL 23
CCL23
CCL23(30-99)
CCL23_HUMAN
Chemokine (C C motif) ligand 23
Chemokine CC motif ligand 23
ck beta 8
Expand