Human IL-4 enzyme-linked immunoassay kit
Catalog# EH0007
Human IL-4 enzyme-linked immunoassay kit
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ELISA
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Human
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1.37~1000 pg/ml
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unconjugated
Overview
Product Name
Human IL-4 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.03 pg/ml (50 μl) ；0.28 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0 | 0.0089 | 0.0085 | 0.0087 | |
| 1.37 | 0.0163 | 0.0168 | 0.0166 | 0.0079 |
| 4.12 | 0.031 | 0.0371 | 0.0341 | 0.0254 |
| 12.35 | 0.0855 | 0.0907 | 0.0881 | 0.0794 |
| 37.04 | 0.2498 | 0.2553 | 0.2526 | 0.2439 |
| 111.11 | 0.7143 | 0.7335 | 0.7239 | 0.7152 |
| 333.33 | 1.9249 | 1.9264 | 1.9257 | 1.917 |
| 1000 | 3.8591 | 3.7755 | 3.8173 | 3.8086 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 24.9 | 124.6 | 243.4 | 28.5 | 126.1 | 219.8 |
| Standard Deviation | 1.3 | 5.5 | 8.2 | 1.9 | 6 | 6.4 |
| Coefficient of Variation (%) | 3.4 | 4.5 | 5.1 | 6.5 | 4.7 | 2.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-4 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 88% to 105% with an overall mean recovery of 98%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-0.49 | 80 | 0.13 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-10 in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
B cell growth factor 1
B cell IgG differentiation factor
B Cell Stimulatory Factor 1
B-cell stimulatory factor 1
BCGF 1
BCGF1
Binetrakin
BSF-1
BSF1
IGG1 induction factor
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