Human MMP-7 enzyme-linked immunoassay kit
Catalog# EH0125
Human MMP-7 enzyme-linked immunoassay kit
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ELISA
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Human
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312.5~20000 pg/ml
Overview
Product Name
Human MMP-7 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
72.45 pg/ml (50 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0753 | 0.0815 | 0.0784 | |
| 312.50 | 0.1392 | 0.1352 | 0.1372 | 0.0588 |
| 625.00 | 0.1928 | 0.1952 | 0.1940 | 0.1156 |
| 1250.00 | 0.3001 | 0.2910 | 0.2956 | 0.2172 |
| 2500.00 | 0.4927 | 0.4717 | 0.4822 | 0.4038 |
| 5000.00 | 0.8668 | 0.8596 | 0.8632 | 0.7848 |
| 10000.00 | 1.6280 | 1.5980 | 1.6130 | 1.5346 |
| 20000.00 | 2.9050 | 2.7370 | 2.8210 | 2.7426 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 490.3 | 2109.8 | 6708.0 | 456.7 | 2055.5 | 6243.6 |
| Standard Deviation | 30.3 | 81.7 | 274.3 | 25.6 | 94.3 | 290.7 |
| Coefficient of Variation (%) | 6.2 | 3.9 | 4.1 | 5.6 | 4.6 | 4.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human MMP-7 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 82% to 108% with an overall mean recovery of 98%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1696.59-7447.73 | 100.0 | 4027.16 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of MMP-7 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Matrilysin
Matrin
Matrix Metalloproteinase 7
Matrix metalloproteinase-7
MMP 7
MMP-7
MMP7
MMP7_HUMAN
MPSL1
PUMP 1
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