Human CCL17/TARC enzyme-linked immunoassay kit
Catalog# EH0164
Human CCL17/TARC enzyme-linked immunoassay kit
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ELISA
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Human
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15.63~1000 pg/ml
Overview
Product Name
Human CCL17/TARC enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
5.67 pg/ml (50 μl)；23.55 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0095 | 0.0089 | 0.0092 | |
| 15.63 | 0.0154 | 0.0164 | 0.0159 | 0.0067 |
| 31.25 | 0.0316 | 0.0331 | 0.0324 | 0.0232 |
| 62.50 | 0.1050 | 0.1019 | 0.1035 | 0.0943 |
| 125.00 | 0.3403 | 0.3721 | 0.3562 | 0.3470 |
| 250.00 | 1.0840 | 1.0790 | 1.0815 | 1.0723 |
| 500.00 | 2.7800 | 2.5100 | 2.6450 | 2.6358 |
| 1000.00 | 4.1525 | 3.9872 | 4.0699 | 4.0607 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 34.6 | 139.7 | 383.4 | 26.0 | 123.2 | 325.1 |
| Standard Deviation | 2.1 | 6.4 | 26.2 | 0.5 | 2.8 | 17.0 |
| Coefficient of Variation (%) | 6.2 | 4.6 | 6.8 | 2.1 | 2.3 | 5.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CCL17/TARC into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 100% to 128% with an overall mean recovery of 115%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 28.6-272.53 | 100 | 110.93 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CCL17/TARC in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
A-152E5.3
A152E53
ABCD 2
ABCD2
C-C motif chemokine 17
CC chemokine TARC
CCL17
CCL17_HUMAN
Chemokine CC Motif Ligand 17
MGC138271
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