Human Prorelaxin H2/RLN2 enzyme-linked immunoassay kit
Catalog# EH0331
Human Prorelaxin H2/RLN2 enzyme-linked immunoassay kit
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sELISA
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Human
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1.37 ~1000 pg/ml
Overview
Product Name
Human Prorelaxin H2/RLN2 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.09 pg/ml (50 μl)；0.80 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0082 | 0.0093 | 0.0088 | |
| 1.37 | 0.0129 | 0.0133 | 0.0131 | 0.0044 |
| 4.12 | 0.0325 | 0.0315 | 0.0320 | 0.0233 |
| 12.35 | 0.0875 | 0.0915 | 0.0895 | 0.0808 |
| 37.04 | 0.2616 | 0.2738 | 0.2677 | 0.2590 |
| 111.11 | 0.7876 | 0.8207 | 0.8042 | 0.7954 |
| 333.33 | 2.0640 | 2.0270 | 2.0455 | 2.0368 |
| 1000.00 | 4.1180 | 4.2850 | 4.2015 | 4.1928 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 21.7 | 116.9 | 329.3 | 21.8 | 115.5 | 331.0 |
| Standard Deviation | 0.6 | 4.2 | 13.8 | 0.4 | 6.5 | 24.3 |
| Coefficient of Variation (%) | 2.7 | 3.6 | 4.2 | 1.6 | 5.6 | 7.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Prorelaxin H2/RLN2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 78% to 129% with an overall mean recovery of 108%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 0.02-53.49 | 100 | 3.87 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human Prorelaxin H2/RLN2 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
H2
REL2_HUMAN
Relaxin 2
Relaxin A chain
Relaxin H2
RLN2
RLXH2