Human Thrombomodulin enzyme-linked immunoassay kit
Catalog# EH0328
Human Thrombomodulin enzyme-linked immunoassay kit
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ELISA
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Human
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156.25 ~10000 pg/ml
Overview
Product Name
Human Thrombomodulin enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
3.73 pg/ml (50 μl)；4.40 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0511 | 0.0490 | 0.0501 | |
| 156.25 | 0.1620 | 0.1604 | 0.1612 | 0.1112 |
| 312.50 | 0.2721 | 0.2656 | 0.2689 | 0.2188 |
| 625.00 | 0.4935 | 0.4966 | 0.4951 | 0.4450 |
| 1250.00 | 0.9292 | 0.9211 | 0.9252 | 0.8751 |
| 2500.00 | 1.7800 | 1.8000 | 1.7900 | 1.7400 |
| 5000.00 | 3.1980 | 3.0140 | 3.1060 | 3.0560 |
| 10000.00 | 4.1872 | 4.2956 | 4.2414 | 4.1914 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 175.0 | 901.0 | 2768.8 | 192.8 | 960.4 | 3060.5 |
| Standard Deviation | 7.4 | 35.0 | 166.9 | 12.4 | 48.9 | 93.9 |
| Coefficient of Variation (%) | 4.2 | 3.9 | 6.0 | 6.4 | 5.1 | 3.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Thrombomodulin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 98% to 112% with an overall mean recovery of 105%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 5246.47-10627.71 | 100 | 8020.10 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human Thrombomodulin in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
AHUS 6 antibody
AHUS6 antibody
BDCA 3 antibody
BDCA3 antibody
CD 141 antibody
CD141 antibody
CD141 antigen antibody
Fetomodulin antibody
Thbd antibody
THPH12 antibody
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