Human HGF enzyme-linked immunoassay kit
Catalog# EH0019
Human HGF enzyme-linked immunoassay kit
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ELISA
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Human
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10.97~8000 pg/ml
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unconjugated
Overview
Product Name
Human HGF enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
4.07 pg/ml (50 μl)；6.01 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0284 | 0.0277 | 0.0281 | |
| 10.97 | 0.0360 | 0.0382 | 0.0371 | 0.0091 |
| 32.92 | 0.0521 | 0.0501 | 0.0511 | 0.0231 |
| 98.77 | 0.1109 | 0.0958 | 0.1034 | 0.0753 |
| 296.30 | 0.2883 | 0.2774 | 0.2829 | 0.2548 |
| 888.89 | 0.8003 | 0.8033 | 0.8018 | 0.7738 |
| 2666.67 | 2.3398 | 2.3380 | 2.3389 | 2.3109 |
| 8000.00 | 3.9661 | 4.0435 | 4.0048 | 3.9768 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 190.1 | 1075.3 | 3244.1 | 214.2 | 1168.4 | 3780.4 |
| Standard Deviation | 12.4 | 73.9 | 219.8 | 15.2 | 69.0 | 144.5 |
| Coefficient of Variation (%) | 6.5 | 6.9 | 6.8 | 7.1 | 5.9 | 3.8 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人HGF，未加人 HGF 的血清作为本底，计算回收率。回收率的范围从 88 % 至 121 %，平均回收率为 108 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 360.14-1072.52 | 100 | 609.53 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
DFNB39
F TCF
Fibroblast derived tumor cytotoxic factor
Hepatocyte growth factor (hepapoietin A
scatter factor)
Hepatocyte growth factor
Hepatocyte growth factor beta chain
Hepatocyte growth factor precursor
Hepatopoietin A
Hepatopoietin-A
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