Human TSLP enzyme-linked immunoassay kit

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Catalog# EH0061

Human TSLP enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

2.74~2000 pg/ml

Conjugation

unconjugated

Overview

Product Name

Human TSLP enzyme-linked immunoassay kit

Product Type

Elisa Kit

Sensitivity

0.03 pg/ml (50 μl)；0.50 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0073	0.0072	0.0073
2.74	0.0340	0.0366	0.0353	0.0281
8.23	0.0859	0.0919	0.0889	0.0817
24.69	0.2592	0.2538	0.2565	0.2493
74.07	0.6801	0.6553	0.6677	0.6605
222.22	1.5230	1.5660	1.5445	1.5373
666.67	2.6030	2.6530	2.6280	2.6208
2000.00	3.2000	3.3710	3.2855	3.2783

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	41.7	204.3	564.7	45.1	213.8	596.2
Standard Deviation	2.0	11.1	27.7	1.9	10.2	29.3
Coefficient of Variation (%)	4.9	5.4	4.9	3.4	2.9	3.0

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human TSLP into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 120% with an overall mean recovery of 103%.

Sample Value

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	n.d.-10.83	33.3	3.99

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of TSLP in this assay. No medical histories were available for the donors.

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

120min

Standard Curve Gradient

7 Points/3 Folds

Incubation Count

Detectable Sample

Dosing Volume

50 μl/10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Target

UNIPROT

SwissProt: Q969D9 Human

Alias

Thymic stromal lymphopoietin

Thymic stromal lymphopoietin protein TSLP

Tslp

TSLP protein

TSLP_HUMAN

Function

Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. The activities of mouse TSLP overlap with, but are distinct from, those of mouse IL-7. Both mouse TSLP and IL-7 can co-stimulate growth of thymocytes and mature T cells, and support B lymphopoiesis in long-term cultures of fetal liver cells and bone-marrow cells. Whereas mouse IL-7 facilitates the development of B220+/IgM- pre-B cells, mouse TSLP promotes the development B220+/IgM+ B cells. Human TSLP was reported to preferentially stimulate myeloid cells, inducing the release of T cell-attracting chemokines from monocytes and enhancing the maturation of CD11c+ dendritic cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence. Within the mature region, six of the seven cysteine residues present in the mouse TSLP involved in intramolecular disulfide bond formation are conserved in the human TSLP. Human TSLP shares approximately 43% aa sequence identity with mouse TSLP. By Northern blot analysis, human TSLP expression has been detected in many tissues with the highest expressions in heart, liver, testis and prostate. TSLP signals through a heterodimeric receptor complex that consists of IL-7 R alpha and the TSLP R, a member of the hemopoietin receptor family most closely related to Rgammac. TSLP R, also named Delta and CRLM-2 (Cytokine Receptor-like Module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain (gamma c). It was subsequently identified as a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R. TSLP R shows 24% sequence identity with the mouse gc receptor. TSLP R expression is constitutive in immune and hematopoietic cells, but is up-regulated in Th2 cells.

Human TSLP enzyme-linked immunoassay kit

Usd: Special Discount

Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Detectable Sample

Dosing Volume

Product Features

Storage Condition

Target

UNIPROT

Alias

Function

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