Human Granzyme K enzyme-linked immunoassay kit
Catalog# EH0301
Human Granzyme K enzyme-linked immunoassay kit
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ELISA
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Human
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4.10~1000 pg/ml
Overview
Product Name
Human Granzyme K enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.36 pg/ml (50 μl)；2.44 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0260 | 0.0248 | 0.0254 | |
| 4.10 | 0.0598 | 0.0552 | 0.0575 | 0.0321 |
| 10.24 | 0.1089 | 0.1120 | 0.1105 | 0.0851 |
| 25.60 | 0.2159 | 0.2080 | 0.2120 | 0.1866 |
| 64.00 | 0.5337 | 0.5264 | 0.5301 | 0.5047 |
| 160.00 | 1.1840 | 1.1190 | 1.1515 | 1.1261 |
| 400.00 | 2.3040 | 2.1600 | 2.2320 | 2.2066 |
| 1000.00 | 3.7910 | 3.7800 | 3.7855 | 3.7601 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 17.2 | 98.6 | 282.3 | 17.2 | 98.8 | 319.5 |
| Standard Deviation | 0.9 | 5.6 | 22.0 | 0.5 | 4.0 | 22.0 |
| Coefficient of Variation (%) | 5.2 | 5.7 | 7.8 | 2.8 | 4.1 | 6.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Granzyme K into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 98% to 127% with an overall mean recovery of 119%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 2.63-35.25 | 100 | 11.74 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human Granzyme K in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃