Human IL-2 enzyme-linked immunoassay kit

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Catalog# EH0013

Human IL-2 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

6.86~5000 pg/ml

Conjugation

unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Human IL-2 enzyme-linked immunoassay kit

Product Type

Elisa Kit

Sensitivity

1.67 pg/ml (50 μl) ；3.08 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0093	0.0085	0.0089
78.13	0.0663	0.0726	0.0695	0.0606
156.25	0.1299	0.1416	0.1358	0.1269
312.50	0.2600	0.2822	0.2711	0.2622
625.00	0.5334	0.5230	0.5282	0.5193
1250.00	1.1330	1.1310	1.1320	1.1231
2500.00	1.8960	1.8960	1.8960	1.8871
5000.00	3.3500	3.3630	3.3565	3.3476

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	92.1	407.5	1540.9	102.1	502.1	1990.9
Standard Deviation	4.7	23.4	86.4	6.0	29.1	119.09
Coefficient of Variation (%)	5.1	5.7	5.6	5.9	5.8	6.0

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested Twenty-two times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human IL-2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 115% with an overall mean recovery of 103%.

Sample Value

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	n.d.	0	0

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-2 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

120min

Standard Curve Gradient

7 Points/3 Folds

Incubation Count

Detectable Sample

Dosing Volume

50 μl/10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Application in Publications

ELISA

See 1 publications below

Application in Publications

Human

See 1 publications below

Target

UNIPROT

SwissProt: P60568 Human

Alias

Aldesleukin

IL 2

IL-2

IL2

IL2_HUMAN

Interleukin 2

Interleukin-2

interleukin2

Involved in regulation of T cell clonal expansion

Lymphokine

Expand

Function

Interleukin 2 (IL-2), also known as T cell growth factor (TCGF), is a 15-18 kDa variably glycosylated alpha -helical polypeptide that is a member of the Common gamma Chain ( gamma c) cytokine family. It exists as a monomer and has a notably short half-life (< 30 minutes). Human IL-2 is synthesized as a 153 amino acid (aa) precursor that contains a 20 aa signal sequence plus a 133 aa mature region. The mature region is alpha -helical in nature, and contains one utilized O-linked glycosylation site at Thr3 plus three cysteines, two of which form an intrachain disulfide bond that is essential for activity. Mature human IL-2 shares 73%, 66%, 78% and 97% aa identity with canine, rat, feline and rhesus monkey IL-2, respectively. Although human IL-2 shares only approximately 60% aa identity with the highly polymorphic mouse IL-2, human IL-2 is known to be active on mouse IL-2 responsive cells. Cells reported to secrete IL-2 include gamma δ T cells, activated conventional CD4+ and CD8+ T cells, neurons, microglia, and hematopoietic stem cells. The receptor for IL-2 (IL-2 R) is composed of three subunits, the 55 kDa CD25/IL-2 R alpha chain, the 70 kDa IL-2 R beta chain, and the 65 kDa Common gamma Chain. IL-2 first binds to CD25, the binary complex then recruits IL-2 R beta and gamma c to form the quaternary signaling complex. In addition to IL-2, IL-2 R beta is used by IL-15 in its quaternary signaling complex. gamma c also serves as a signaling receptor for IL-4, -7, -9, -15, and -21. In vitro studies have shown an important role for IL-2 in T cell activation and expansion. In vivo, IL-2 is critical for the development, maintenance and function of regulatory T cells (Treg) which provide protection against autoimmune disease. On the other hand, IL-2 can also promote autoimmune inflammation in target organs through its roles in regulating the expression of T cell trafficking genes, and production of Th2 cytokines. Within the CD8+ T cell subset, IL-2 is essential for optimal primary responses and differentiation into terminal effector cells. IL-2 also promotes the development of activated CD8+ T cells into memory cells.

Citation

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Filter by Reactivity

A ceramic microbridge microfluidic chip to study osteogenic differentiation of mesenchymal stem cells in bioactive ceramic immune microenvironment
- Journal: Bioactive Materials
- DOI: 10.1016/j.bioactmat.2024.11.005
- IF: 18
- Application: ELISA
- Reactivity: Human
- Publish date: 2024 Dec

Human IL-2 enzyme-linked immunoassay kit

Usd: Special Discount

Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Detectable Sample

Dosing Volume

Product Features

Storage Condition

Application in Publications

Application in Publications

Target

UNIPROT

Alias

Function

Citation

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