Human IL-13R alpha 2 enzyme-linked immunoassay kit
Catalog# EH0182
Human IL-13R alpha 2 enzyme-linked immunoassay kit
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ELISA
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Human
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27.43~20000 pg/ml
Overview
Product Name
Human IL-13R alpha 2 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
3.50 pg/ml (50 μl)；22.29 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0106 | 0.0100 | 0.0103 | |
| 27.43 | 0.0238 | 0.0228 | 0.0233 | 0.0130 |
| 82.30 | 0.0507 | 0.0475 | 0.0491 | 0.0388 |
| 246.91 | 0.1314 | 0.1236 | 0.1275 | 0.1172 |
| 740.74 | 0.3798 | 0.3780 | 0.3789 | 0.3686 |
| 2222.22 | 1.0320 | 1.0130 | 1.0225 | 1.0122 |
| 6666.67 | 2.5480 | 2.5080 | 2.5280 | 2.5177 |
| 20000.00 | 4.1779 | 4.1531 | 4.1655 | 4.1552 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 372.5 | 1840.8 | 6053.0 | 379.6 | 1922.9 | 6355.7 |
| Standard Deviation | 20.6 | 126.5 | 290.5 | 18.2 | 110.3 | 206.8 |
| Coefficient of Variation (%) | 5.5 | 6.9 | 4.8 | 4.8 | 5.7 | 3.3 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 IL-13 R alpha 2，未加人 IL-13 R alpha 2 的血清作为本底，计算回收率。回收率的范围从 80 % 至 105 %，平均回收率为 87 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-23.65 | 60 | 8.9 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
CD213a2 antigen
CD213a2
CT19
IL-13 R alpha 2
IL-13 receptor subunit alpha-2
cancer/testis antigen 19
IL13BP
IL13R alpha 2
IL-13R subunit alpha-2
IL13R
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