Human IGFBP3/IBP3 enzyme-linked immunoassay kit(one step)
Catalog# EHY0157
Human IGFBP3/IBP3 enzyme-linked immunoassay kit(one step)
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ELISA
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Human
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0.27~200 ng/ml
Overview
Product Name
Human IGFBP3/IBP3 enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.004 ng/ml (50 μl)；13.60 ng/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0115 | 0.0102 | 0.0109 | |
| 0.27 | 0.0451 | 0.0421 | 0.0436 | 0.0328 |
| 0.82 | 0.1053 | 0.1027 | 0.1040 | 0.0932 |
| 2.47 | 0.2837 | 0.2744 | 0.2791 | 0.2682 |
| 7.41 | 0.7716 | 0.7461 | 0.7589 | 0.7480 |
| 22.22 | 1.8110 | 1.7790 | 1.7950 | 1.7842 |
| 66.67 | 3.2530 | 3.0140 | 3.1335 | 3.1227 |
| 200.00 | 4.1758 | 3.9680 | 4.0719 | 4.0611 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 5.7 | 19.0 | 65.8 | 4.0 | 13.7 | 54.5 |
| Standard Deviation | 0.3 | 1.1 | 4.0 | 0.2 | 0.7 | 3.0 |
| Coefficient of Variation (%) | 4.7 | 5.9 | 6.0 | 3.9 | 5.0 | 5.5 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 IGFBP3/IBP3，未加人 IGFBP3/IBP3 的血清作为本底，计算回收率。回收率的范围从 80 % 至 107 %，平均回收率为 89%。
Sample Value
| Sample Matrix | Evaluated Sample | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 3.82-8.78 | 100 | 5.74 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Acid stable subunit of the 140 K IGF complex
Binding protein 29
Binding protein 53
BP 53
BP53
Growth hormone dependent binding protein
IBP 3
IBP-3
IBP3
IBP3_HUMAN
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