Human Oxytocin-neurophysin 1/OXT enzyme-linked immunoassay kit
Catalog# EH0153
Human Oxytocin-neurophysin 1/OXT enzyme-linked immunoassay kit
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ELISA
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Human
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40.96~10000 pg/ml
Overview
Product Name
Human Oxytocin-neurophysin 1/OXT enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
1.02 pg/ml (50 μl)；4.22 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0068 | 0.0081 | 0.0075 | |
| 40.96 | 0.0280 | 0.0282 | 0.0281 | 0.0207 |
| 102.40 | 0.0630 | 0.0590 | 0.0610 | 0.0536 |
| 256.00 | 0.1461 | 0.1605 | 0.1533 | 0.1459 |
| 640.00 | 0.4384 | 0.4124 | 0.4254 | 0.4180 |
| 1600.00 | 1.1440 | 1.1410 | 1.1425 | 1.1351 |
| 4000.00 | 2.9610 | 2.8740 | 2.9175 | 2.9101 |
| 10000.00 | 4.0079 | 4.1190 | 4.0635 | 4.0560 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 218.3 | 1133.2 | 3538.1 | 221.8 | 1122.4 | 3350.1 |
| Standard Deviation | 6.8 | 40.5 | 130.7 | 6.3 | 60.8 | 184.5 |
| Coefficient of Variation (%) | 3.1 | 3.6 | 3.7 | 2.8 | 5.4 | 5.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Oxytocin-neurophysin 1/OXT into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 95% to 119% with an overall mean recovery of 103%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 23.53-1127.81 | 100 | 175.25 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Oxytocin-neurophysin 1/OXT in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
MGC126890
MGC126892
Neurophysin 1
Neurophysin I
Ocytocin
OT
OT NPI
OXT
Oxytocin
Oxytocin Neurophysin preproprotein
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