Human Granzyme B enzyme-linked immunoassay kit

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Catalog# EH0052

Human Granzyme B enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

78.13~5000 pg/ml

Conjugation

unconjugated

Overview

Product Name

Human Granzyme B enzyme-linked immunoassay kit

Product Type

Elisa Kit

Sensitivity

6.62 pg/ml (50 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0354	0.0305	0.0330
78.13	0.0862	0.0936	0.0899	0.0570
156.25	0.1339	0.1493	0.1416	0.1087
312.50	0.2451	0.2431	0.2441	0.2112
625.00	0.3759	0.3953	0.3856	0.3527
1250.00	0.7970	0.7870	0.7920	0.7591
2500.00	1.4500	1.3650	1.4075	1.3746
5000.00	2.4690	2.3120	2.3905	2.3576

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	97.1	448.0	1618.5	84.9	453.4	1514.2
Standard Deviation	5.1	17.4	58.5	3.7	10.0	31.0
Coefficient of Variation (%)	5.2	3.9	3.6	4.3	2.2	2.1

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human Granzyme B into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 101% to 128% with an overall mean recovery of 111%.

Sample Value

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	n.d.-58.36	37.5	14.21

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Granzyme B in this assay. No medical histories were available for the donors.

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

120min

Standard Curve Gradient

7 Points/3 Folds

Incubation Count

Detectable Sample

Dosing Volume

50 μl/10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Target

UNIPROT

SwissProt: P10144 Human

Alias

C11

Cathepsin G like 1

Cathepsin G-like 1

CCPI

CGL 1

CGL1

CSP B

CSPB

CTLA 1

CTLA-1

Expand

Function

Granzyme B is a member of the granzyme family of serine proteases found specifically in granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme B plays an essential role in granule-mediated apoptosis utilizing the substrates in this pathway, such as Caspase 3, Caspase 8 and Bid. Recent research indicates expanded Granzyme B functionality to include extracellular roles along with its classical pro-apoptotic function. It has been found that Granzyme B is an important mediator of skin injury, repair and inflammation through extracellular substrates including Laminin, VE-Cadherin, Fibronectin and the proteoglycans Aggrecan and Decorin. As one of the five Granzymes (A, B, H, K and M) identified in the human genome, Granzyme B (32kDa) is the most widely researched in terms of its biological function and its utility in health and disease . It is synthesized as a precursor (247 residues) with a signal peptide (residues 1-18), a pro-peptide (residues 19-20), and a mature chain (residues 21-247). Once inside granules, Granzyme B is fully processed into the mature chain and becomes an active protease when the pro-peptide, Gly-Glu is removed from the N-terminus by cleavage with Cathepsin C. The protease activity of Granzyme B is tightly controlled by Serpin B9/ Protease Inhibitor 9. The amino acid sequence of human Granzyme B is 71%, 69%, and 68% identical to its canine, rat, and mouse counterparts, respectively. Granzymes have been shown to modulate inflammation, and Granzyme B plasma levels have been found higher with atopic dermatitis and psoriasis when compared to healthy controls. This is in contrast to Granzyme A plasma levels which remain unchanged. Serum from patients with Crohn's disease have significantly higher Granzyme B levels than controls.

Human Granzyme B enzyme-linked immunoassay kit

Usd: Special Discount

Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Detectable Sample

Dosing Volume

Product Features

Storage Condition

Target

UNIPROT

Alias

Function

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