Human Myeloperoxidase(MPO) enzyme-linked immunoassay kit(one step)
Catalog# EHY0205
Human Myeloperoxidase(MPO) enzyme-linked immunoassay kit(one step)
-
ELISA
-
Human
-
0.14~100 ng/ml
Overview
Product Name
Human Myeloperoxidase(MPO) enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.02 ng/ml（50 μl）；0.07 ng/ml（10 μl）
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0088 | 0.0099 | 0.0094 | |
| 0.14 | 0.0226 | 0.0236 | 0.0231 | 0.0138 |
| 0.41 | 0.0502 | 0.0532 | 0.0517 | 0.0424 |
| 1.23 | 0.1425 | 0.1269 | 0.1347 | 0.1254 |
| 3.70 | 0.4044 | 0.4130 | 0.4087 | 0.3994 |
| 11.11 | 1.1640 | 1.1530 | 1.1585 | 1.1492 |
| 33.33 | 2.9250 | 3.0390 | 2.9820 | 2.9727 |
| 100.00 | 4.4116 | 4.5568 | 4.4842 | 4.4749 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 1.9 | 9.1 | 34.0 | 1.8 | 9.2 | 33.6 |
| Standard Deviation | 0.1 | 0.3 | 1.6 | 0.1 | 0.3 | 0.8 |
| Coefficient of Variation (%) | 3.1 | 3.7 | 4.7 | 4.7 | 2.9 | 2.4 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 Myeloperoxidase，未加人 Myeloperoxidase 的血清作为本底，计算回收率。回收率的范围从 95 % 至 117 %，平均回收率为 109%。
Sample Value
| Sample Matrix | Evaluated Sample | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 3.72-19.70 | 100 | 7.8 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
84 kDa myeloperoxidase
89 kDa myeloperoxidase
EC 1.11.1.7
EC1.11.2.2
fj80f04
MPO
mpx
myeloid-specific peroxidase
Myeloperoxidase
Myeloperoxidase heavy chain
Expand