Human LAG-3 enzyme-linked immunoassay kit

Usd: 619 Special Discount

Specification

96T

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Catalog# EH0005

Human LAG-3 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

156.25~10000 pg/ml

Overview

Product Name

Human LAG-3 enzyme-linked immunoassay kit

Product Type

Elisa Kit

Sensitivity

3.40 pg/ml (50 μl) ；36.81 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0152	0.0139	0.0139
156.25	0.0949	0.0900	0.0925	0.0786
312.50	0.1725	0.1668	0.1697	0.1558
625.00	0.3285	0.3315	0.3300	0.3161
1250.00	0.6285	0.6328	0.6307	0.6168
2500.00	1.1310	1.1440	1.1375	1.1236
5000.00	1.9550	2.0120	1.9835	1.9696
10000.00	3.0790	3.0990	3.0890	3.0751

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	222.9	1049.2	3198.7	190.1	934.2	2834.4
Standard Deviation	8.9	31.4	153.4	7.3	20.1	92.9
Coefficient of Variation (%)	4.0	3.0	4.8	3.9	2.1	3.3

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human LAG-3 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 118% with an overall mean recovery of 95%.

Sample Value

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	474.27-6835.60	100	2298.95

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human LAG-3 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

120min

Standard Curve Gradient

7 Points

Incubation Count

Detectable Sample

Dosing Volume

50 μl/10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Target

UNIPROT

SwissProt: P18627 Human

Function

LAG-3 (Lymphocyte activation gene-3), also known as CD223, is a member of the immunoglobulin superfamily (IgSF). The mature LAG-3 protein is a 496 amino acid (aa) membrane protein with a 421 aa extracellular region which contains four IgSF domains, a 21 aa transmembrane region and a 54 aa cytoplasmic region. The extracellular domain of human and mouse LAG-3 share 69% amino acid sequence identity. LAG-3 and CD4 molecules share 20% aa sequence homology but have a similar structure. Both molecules bind to MHC class II. LAG-3 binds to MHC class II with higher affinity compared to CD4. LAG-3 is an activation-induced molecule, expressed on activated T cells and NK cells, but not on resting T cells. Studies using LAG-3 -/- mice have shown significant delay of T cell apoptosis following antigen stimulation and increased size of memory T cells pool following infection. It also has been reported that anti-LAG-3 antibodies up-regulate T cell activation by blocking interaction of LAG-3 and MHC class II. The study has demonstrated that LAG-3 is selectively expressed on activated CD4+CD25+ TReg cells and plays a role in their suppressive activity. This evidence indicated, unlike the interaction of CD4 with MHC class II that plays a positive role in T cell activation, LAG-3 binds to MHC class II and negatively regulates T cell activation through LAG-3 signaling. On the other hand, studies have shown that binding of LAG-3 to MHC class II molecules on antigen presenting cells induce maturation of dendritic cells and cytokine secretion by monocytes through MHC class II signal transduction. Taken together, LAG-3 may have two major functions, it negatively regulates T cells activation through LAG-3 signaling and stimulates antigen presenting cells which express MHC class II.

Human LAG-3 enzyme-linked immunoassay kit

Usd: 619 Special Discount

Specification

Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Detectable Sample

Dosing Volume

Product Features

Storage Condition

Target

UNIPROT

Function

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