Human TNF beta/Lymphotoxin alpha enzyme-linked immunoassay kit
Catalog# EH0181
Human TNF beta/Lymphotoxin alpha enzyme-linked immunoassay kit
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ELISA
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Human
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40.96~10000 pg/ml
Overview
Product Name
Human TNF beta/Lymphotoxin alpha enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
10.59 pg/ml (50 μl)；83.08 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0139 | 0.0137 | 0.0138 | |
| 40.96 | 0.0270 | 0.0261 | 0.0266 | 0.0128 |
| 102.40 | 0.0495 | 0.0454 | 0.0475 | 0.0337 |
| 256.00 | 0.1006 | 0.0943 | 0.0975 | 0.0837 |
| 640.00 | 0.2308 | 0.2137 | 0.2223 | 0.2085 |
| 1600.00 | 0.5287 | 0.4955 | 0.5121 | 0.4983 |
| 4000.00 | 1.2490 | 1.1550 | 1.2020 | 1.1882 |
| 10000.00 | 2.4840 | 2.4060 | 2.4450 | 2.4312 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 326.8 | 1415.9 | 4105.8 | 226.7 | 1146.4 | 3807.0 |
| Standard Deviation | 21.7 | 86.6 | 262.9 | 8.1 | 65.9 | 68.6 |
| Coefficient of Variation (%) | 6.6 | 6.1 | 6.4 | 3.6 | 5.7 | 1.8 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 TNF beta/Lymphotoxin alpha，未加人 TNF beta/Lymphotoxin alpha 的血清作为本底，计算回收率。回收率的范围从 92 % 至 117 %，平均回收率为 105 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-31.50 | 25 | 16.64 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
LTalpha
DIF
LT alpha
LT
LT-alpha
Lta
Lymphotoxin alpha (TNF superfamily, member 1)
Lymphotoxin alpha
Lymphotoxin-alpha
TNF B
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