Human TGF-α enzyme-linked immunoassay kit
Catalog# EH0059
Human TGF-α enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
4.12~3000 pg/ml
Conjugation
-
unconjugated
Overview
Product Name
Human TGF-α enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
1.91 pg/ml (50 μl)；3.89 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0422 | 0.0382 | 0.0402 | |
| 4.12 | 0.0471 | 0.0527 | 0.0499 | 0.0439 |
| 12.35 | 0.0726 | 0.0712 | 0.0719 | 0.0659 |
| 37.04 | 0.1285 | 0.1285 | 0.1285 | 0.1225 |
| 111.11 | 0.3091 | 0.3135 | 0.3113 | 0.3053 |
| 333.33 | 0.7566 | 0.7792 | 0.7679 | 0.7619 |
| 1000.00 | 1.7560 | 1.6690 | 1.7125 | 1.7065 |
| 3000.00 | 3.1350 | 3.1530 | 3.1440 | 3.1380 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 59.6 | 296.2 | 1122.0 | 58.2 | 320.6 | 1180.8 |
| Standard Deviation | 2.7 | 20.8 | 74.4 | 2.3 | 16.3 | 40.8 |
| Coefficient of Variation (%) | 4.5 | 7.0 | 6.6 | 4.0 | 5.1 | 3.5 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人TGF-α，未加人 TGF-α 的血清作为本底，计算回收率。回收率的范围从 104 % 至 126 %，平均回收率为 114 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-25.28 | 50 | 9.57 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
EGF like TGF
EGF-like TGF
ETGF
TFGA
TGF A
TGF type 1
TGF-alpha
Tgfa
TGFA_HUMAN
Transforming growth factor alpha
ExpandFunction
TGF-α是EGF细胞因子家族的成员，其合成为跨膜前体，通过蛋白水解裂解释放可溶性形式。膜结合的proTGF-α具有生物活性，在介导细胞间粘附和相邻细胞的并列刺激中发挥作用。TGF-α的表达在肿瘤和转化细胞中广泛存在。TGF-α在胚胎发生过程中也在正常组织和成人组织中表达，包括垂体，脑，角质形成细胞和巨噬细胞。TGF-α结合并激活EGF受体。它诱导成纤维细胞增殖、上皮发育、角质形成细胞迁移和血管生成。