Human IKK gamma/NEMO enzyme-linked immunoassay kit
Catalog# EH0324
Human IKK gamma/NEMO enzyme-linked immunoassay kit
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ELISA
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Human
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31.25~2000 pg/ml
Overview
Product Name
Human IKK gamma/NEMO enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
2.0 pg/ml (50 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0357 | 0.0330 | 0.0344 | |
| 31.25 | 0.0975 | 0.0863 | 0.0919 | 0.0576 |
| 62.50 | 0.1553 | 0.1381 | 0.1467 | 0.1124 |
| 125.00 | 0.2683 | 0.2467 | 0.2575 | 0.2232 |
| 250.00 | 0.5123 | 0.4686 | 0.4905 | 0.4561 |
| 500.00 | 0.9761 | 0.9020 | 0.9391 | 0.9047 |
| 1000.00 | 1.8510 | 1.6800 | 1.7655 | 1.7312 |
| 2000.00 | 3.3560 | 3.1000 | 3.2280 | 3.1937 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 25.5 | 161.0 | 521.3 | 25.4 | 164.0 | 528.7 |
| Standard Deviation | 1.1 | 4.6 | 11.1 | 2.0 | 6.7 | 17.6 |
| Coefficient of Variation (%) | 4.3 | 2.8 | 2.1 | 7.9 | 4.1 | 3.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IKK gamma/NEMO into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 75% to 95% with an overall mean recovery of 82%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 15.39-70.98 | 100 | 37.33 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human IKK gamma/NEMO in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃