Human SCF/KITLG enzyme-linked immunoassay kit
Catalog# EH0174
Human SCF/KITLG enzyme-linked immunoassay kit
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ELISA
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Human
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6.86~5000 pg/ml
Overview
Product Name
Human SCF/KITLG enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.22 pg/ml (50 μl)；2.44 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0153 | 0.0131 | 0.0142 | |
| 6.86 | 0.0424 | 0.0400 | 0.0412 | 0.0270 |
| 20.58 | 0.0919 | 0.0914 | 0.0917 | 0.0775 |
| 61.73 | 0.2355 | 0.2313 | 0.2334 | 0.2192 |
| 185.19 | 0.6456 | 0.6603 | 0.6530 | 0.6388 |
| 555.56 | 1.6390 | 1.6610 | 1.6500 | 1.6358 |
| 1666.67 | 3.0210 | 3.1300 | 3.0755 | 3.0613 |
| 5000.00 | 3.9070 | 4.0151 | 3.9611 | 3.9469 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 107.7 | 508.8 | 1485.5 | 112.3 | 503.0 | 1608.2 |
| Standard Deviation | 6.1 | 27.0 | 105.1 | 7.0 | 30.9 | 115.0 |
| Coefficient of Variation (%) | 5.6 | 5.3 | 7.1 | 6.2 | 6.1 | 7.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human SCF/KITLG into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 71% to 90% with an overall mean recovery of 82%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1574.28-4366.78 | 100 | 2624.25 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of SCF/KITLG in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
C kit ligand
C-kit ligand
Ckit ligand
DCUA
DFNA69
DKFZp686F2250
familial progressive hyperpigmentation 2
FPH2
FPHH
KIT ligand
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