Human Fibromodulin/FMOD enzyme-linked immunoassay kit
Catalog# EH0321
Human Fibromodulin/FMOD enzyme-linked immunoassay kit
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sELISA
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Human
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156.25 ~10000 pg/ml
Overview
Product Name
Human Fibromodulin/FMOD enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
8.30 pg/ml (50 μl); 24.10 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0480 | 0.0477 | 0.0479 | |
| 156.25 | 0.1354 | 0.1293 | 0.1324 | 0.0845 |
| 312.50 | 0.2258 | 0.2170 | 0.2214 | 0.1736 |
| 625.00 | 0.4013 | 0.3988 | 0.4001 | 0.3522 |
| 1250.00 | 0.6911 | 0.6603 | 0.6757 | 0.6279 |
| 2500.00 | 1.3290 | 1.2810 | 1.3050 | 1.2572 |
| 5000.00 | 2.1740 | 2.1420 | 2.1580 | 2.1102 |
| 10000.00 | 3.1180 | 3.1410 | 3.1295 | 3.0817 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 142.6 | 880.0 | 2739.3 | 159.5 | 932.2 | 2990.4 |
| Standard Deviation | 7.4 | 23.5 | 129.3 | 4.9 | 54.4 | 210.5 |
| Coefficient of Variation (%) | 5.2 | 2.7 | 4.7 | 3.9 | 2.1 | 3.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Fibromodulin/FMOD into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 105% with an overall mean recovery of 94%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 4267.67-10636.17 | 100 | 7817 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human Fibromodulin/FMOD in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Fibromodulin
FM
Collagen-binding 59 kDa protein
Keratan sulfate proteoglycan fibromodulin
KSPG fibromodulin
FMOD
SLRR2E
FibromodulinFMOD