Human Prostate specific Antigen/KLK3 enzyme-linked immunoassay kit
Catalog# EH0234
Human Prostate specific Antigen/KLK3 enzyme-linked immunoassay kit
-
ELISA
-
Human
-
1.37~1000 pg/ml
Overview
Product Name
Human Prostate specific Antigen/KLK3 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.01 pg/ml (50 μl)；1.17 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0113 | 0.0123 | 0.0118 | |
| 1.37 | 0.0313 | 0.0287 | 0.0300 | 0.0182 |
| 4.12 | 0.0555 | 0.0583 | 0.0569 | 0.0451 |
| 12.35 | 0.1327 | 0.1522 | 0.1425 | 0.1307 |
| 37.04 | 0.4008 | 0.3970 | 0.3989 | 0.3871 |
| 111.11 | 0.9790 | 0.9796 | 0.9793 | 0.9675 |
| 333.33 | 2.1300 | 2.0950 | 2.1125 | 2.1007 |
| 1000.00 | 3.1530 | 3.1460 | 3.1495 | 3.1377 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 22.47 | 110.77 | 353.24 | 26.24 | 124.90 | 268.61 |
| Standard Deviation | 0.59 | 3.83 | 13.52 | 0.84 | 2.86 | 13.52 |
| Coefficient of Variation (%) | 2.6 | 3.5 | 3.8 | 3.2 | 2.3 | 5.0 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 Prostate specific Antigen/KLK3，未加人 Prostate specific Antigen/KLK3 的血清作为本底，计算回收率。回收率的范围从 105 % 至 125 %，平均回收率为 115 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-169.70 | 93.75 | 13.29 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
antigen, prostate-specific
APS
Gamma seminoprotein
Gamma-seminoprotein
hK3
Kallikrein 3
Kallikrein related peptidase 3
Kallikrein-3
KLK 3
KLK2A1
Expand