Human Prostate specific Antigen/KLK3 enzyme-linked immunoassay kit
Catalog# EH0234
Human Prostate specific Antigen/KLK3 enzyme-linked immunoassay kit
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ELISA
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Human
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1.37~1000 pg/ml
Overview
Product Name
Human Prostate specific Antigen/KLK3 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.01 pg/ml (50 μl)；1.17 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0113 | 0.0123 | 0.0118 | |
| 1.37 | 0.0313 | 0.0287 | 0.0300 | 0.0182 |
| 4.12 | 0.0555 | 0.0583 | 0.0569 | 0.0451 |
| 12.35 | 0.1327 | 0.1522 | 0.1425 | 0.1307 |
| 37.04 | 0.4008 | 0.3970 | 0.3989 | 0.3871 |
| 111.11 | 0.9790 | 0.9796 | 0.9793 | 0.9675 |
| 333.33 | 2.1300 | 2.0950 | 2.1125 | 2.1007 |
| 1000.00 | 3.1530 | 3.1460 | 3.1495 | 3.1377 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 22.47 | 110.77 | 353.24 | 26.24 | 124.90 | 268.61 |
| Standard Deviation | 0.59 | 3.83 | 13.52 | 0.84 | 2.86 | 13.52 |
| Coefficient of Variation (%) | 2.6 | 3.5 | 3.8 | 3.2 | 2.3 | 5.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Prostate specific Antigen/KLK3 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 105% to 125% with an overall mean recovery of 115%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-169.70 | 93.75 | 13.29 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Prostate specific Antigen/KLK3 in this assay. No medical histories were available for the donors.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
antigen, prostate-specific
APS
Gamma seminoprotein
Gamma-seminoprotein
hK3
Kallikrein 3
Kallikrein related peptidase 3
Kallikrein-3
KLK 3
KLK2A1
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