Human Siglec 9 enzyme-linked immunoassay kit

Usd: 499 Special Discount

Specification

96T

Bulk Order Quote | Customization Request

Catalog# EH0161

Human Siglec 9 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

10.97~8000 pg/ml

Overview

Product Name

Human Siglec 9 enzyme-linked immunoassay kit

Product Type

Elisa Kit

Sensitivity

1.09 pg/ml (50 μl)；7.36 pg/ml (10 μl)；

pg/ml	O.D.		Average	Corrected
0.00	0.0125	0.0106	0.0116
10.97	0.0198	0.0185	0.0192	0.0076
32.92	0.0372	0.0360	0.0366	0.0251
98.77	0.0902	0.0884	0.0893	0.0778
296.30	0.2258	0.2385	0.2322	0.2206
888.89	0.6742	0.6647	0.6695	0.6579
2666.67	1.7480	1.6900	1.7190	1.7075
8000.00	3.1690	3.3390	3.2540	3.2425

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	149.7	839.3	2679.4	130.7	746.7	2518.4
Standard Deviation	7.2	61.3	183.3	9.7	54.8	156.2
Coefficient of Variation (%)	4.8	7.3	6.8	7.4	7.3	6.2

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human Siglec 9 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 71% to 94% with an overall mean recovery of 78%.

Sample Value

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	2604.82-5014.68	100	3556.41

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Siglec 9 in this assay. No medical histories were available for the donors.

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

120min

Standard Curve Gradient

7 Points/3 Folds

Incubation Count

Detectable Sample

Dosing Volume

50 μl/10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Target

UNIPROT

SwissProt: Q9Y336 Human

Alias

CD329

CDw329

FOAP-9

OBBP-Like

Protein FOAP-9

sialic acid binding Ig-like lectin 9

sialic acid-binding Ig-like lectin 9

Siglec9

Siglec-9

Function

Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains. Eleven human Siglecs have been cloned and characterized. They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglec-5 to -11. To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system. This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasmic tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system. The cDNA of human Siglec-9 encodes a 463 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-likeV-type domain, two Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail. In peripheral blood leukocytes, Siglec-9 is expressed on neutrophils, monocytes, a fraction of NK cells, B cells, and a minor subset of CD8+ T cells. It binds equally well to both 2,3- and 2,6-linked sialic acid. Siglec-9 is closely related to Siglec-7, and they share ~80% amino acid sequence identity. The gene encoding siglec-9 was mapped to chromosome 19q13.4.

Human Siglec 9 enzyme-linked immunoassay kit

Usd: 499 Special Discount

Specification

Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Detectable Sample

Dosing Volume

Product Features

Storage Condition

Target

UNIPROT

Alias

Function

Same Target and Pathway Products

Human IFN-β enzyme-linked immunoassay kit

Human ULBP1/NKG2DL1 enzyme-linked immunoassay kit

Human CD163 enzyme-linked immunoassay kit

Human Renin enzyme-linked immunoassay kit

Human/Mouse MASP2 enzyme-linked immunoassay kit

Human IL-6 Super Sensitivity enzyme-linked immunoassay kit

Human/Monkey Complement C3 enzyme-linked immunoassay kit(one step)

Lentivirus HIV-1 p24 enzyme-linked immunoassay kit（one step）

Human CD273/B7-DC/PD-L2 enzyme-linked immunoassay kit（one step）

Human Complement factor B enzyme-linked immunoassay kit(one step)

Human SNAP25 enzyme-linked immunoassay kit

Human CX3CL1/Fractalking enzyme-linked immunoassay kit

Human CXCL2/MIP2/GRO beta enzyme-linked immunoassay kit

Human tPA enzyme-linked immunoassay kit(one step)

Human IL-3 enzyme-linked immunoassay kit

Human Myeloperoxidase(MPO) enzyme-linked immunoassay kit(one step)

Human B7-H6/NCR3LG1 enzyme-linked immunoassay kit

Human CD48 enzyme-linked immunoassay kit

Human MMP-7 enzyme-linked immunoassay kit

Human CTLA-4 enzyme-linked immunoassay kit

Human/Monkey/Canine CCL5/RANTES enzyme-linked immunoassay kit(one step)

Human Lipoprotein A/LPA enzyme-linked immunoassay kit(one step)

Human CTGF/CCN2 enzyme-linked immunoassay kit(one step)

Human Proprotein Convertase 9/PCSK9 enzyme-linked immunoassay kit（one step）

Human CCL2/MCP-1 enzyme-linked immunoassay kit

Human CCL5/RANTES enzyme-linked immunoassay kit

Human FGF-19 enzyme-linked immunoassay kit

Human Antithrombin III/AT III enzyme-linked immunoassay kit(one step)

Human CD73 enzyme-linked immunoassay kit

Human CD47 enzyme-linked immunoassay kit

Human Neuropilin-1/NRP1 enzyme-linked immunoassay kit（one step）

Human IL-17E/IL-25 enzyme-linked immunoassay kit

Human/Monkey/Canine CXCL10/IP-10 enzyme-linked immunoassay kit

Human/Monkey/Canine Osteopontin（OPN） enzyme-linked immunoassay kit

Human Total HSP60 enzyme-linked immunoassay kit

Human IL-13R alpha 2 enzyme-linked immunoassay kit

Human CHIT1 enzyme-linked immunoassay kit

Human RBP4 enzyme-linked immunoassay kit（one step）

Human Caspase-9 enzyme-linked immunoassay kit

Human SAA1 enzyme-linked immunoassay kit

Human/Monkey Albumin enzyme-linked immunoassay kit(one step)

Human/Monkey PCSK9 enzyme-linked immunoassay kit (one step)

Human TGF-α enzyme-linked immunoassay kit

Human Leptin enzyme-linked immunoassay kit

Human CD27/TNFRSF7 enzyme-linked immunoassay kit

Human MERTK enzyme-linked immunoassay kit（one step）

Human ICAM-1 / CD54 enzyme-linked immunoassay kit

Human/Monkey/Canine IL-18 enzyme-linked immunoassay kit

Human Endoglin/CD105 enzyme-linked immunoassay kit

Human IL-1RA/IL-1F3 enzyme-linked immunoassay kit

Human beta-NGF enzyme-linked immunoassay kit

Human IL-15 enzyme-linked immunoassay kit

Human PAI-1/Serpin E1 enzyme-linked immunoassay kit(one step)

Human CD28 enzyme-linked immunoassay kit

Human CCL25 enzyme-linked immunoassay kit