Human Serpin A12 enzyme-linked immunoassay kit
Catalog# EH0325
Human Serpin A12 enzyme-linked immunoassay kit
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sELISA
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Human
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31.25 ~2000 pg/ml
Overview
Product Name
Human Serpin A12 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
2.48 pg/ml (50 μl)；19.23pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0779 | 0.0784 | 0.0782 | |
| 31.25 | 0.1476 | 0.1496 | 0.1486 | 0.0705 |
| 62.50 | 0.2088 | 0.2223 | 0.2156 | 0.1374 |
| 125.00 | 0.3183 | 0.3407 | 0.3295 | 0.2514 |
| 250.00 | 0.5592 | 0.5849 | 0.5721 | 0.4939 |
| 500.00 | 0.9686 | 1.0220 | 0.9953 | 0.9172 |
| 1000.00 | 1.5950 | 1.6860 | 1.6405 | 1.5624 |
| 2000.00 | 2.6530 | 2.7610 | 2.7070 | 2.6289 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 26.4 | 202.7 | 639.4 | 31.6 | 191.2 | 557.5 |
| Standard Deviation | 2.0 | 14.5 | 46.7 | 2.2 | 7.9 | 28.1 |
| Coefficient of Variation (%) | 7.5 | 7.1 | 7.3 | 7.0 | 4.1 | 5.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Serpin A12 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 70% to 95% with an overall mean recovery of 80%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 6.64-218.03 | 100 | 48.88 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human Serpin A12 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
ENSG00000165953
OL 64
OL-64
OL64
Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 12
Serpin A12
Serpin A12 precursor
Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 12
Serpina12
SPA12_HUMAN
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