Human Endostatin/COL18A1 enzyme-linked immunoassay kit(one step)

Usd: 619 Special Discount

Specification

96T

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Catalog# EHY0232

Human Endostatin/COL18A1 enzyme-linked immunoassay kit(one step)

Application

ELISA

Reactivity

Human

Standard curve range

1.37~1000 ng/ml

Overview

Product Name

Human Endostatin/COL18A1 enzyme-linked immunoassay kit(one step)

Product Type

Elisa Kit

Sensitivity

0.31 ng/ml (10 μl)

ng/ml	O.D.		Average	Corrected
0.00	0.0066	0.0063	0.0065
1.37	0.0117	0.0122	0.0120	0.0055
4.12	0.0255	0.0263	0.0259	0.0195
12.35	0.0680	0.0758	0.0719	0.0655
37.04	0.2051	0.2193	0.2122	0.2058
111.11	0.7228	0.6482	0.6855	0.6791
333.33	1.8520	1.8470	1.8495	1.8431
1000.00	2.8380	2.9900	2.9140	2.9076

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (ng/ml)	18.6	90.3	323.1	25.8	108.2	374.8
Standard Deviation	0.8	3.7	11.3	1.1	4.7	18.7
Coefficient of Variation (%)	4.4	4.1	3.5	4.2	4.3	5.0

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human Endostatin/COL18A1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 95% to 120% with an overall mean recovery of 112%.

Sample Value

Sample Matrix	Sample Evaluated	Range (ng/ml)	Detectable (%)	Mean of Detectable (ng/ml)
Serum	30	58.87-119.93	100	76.55

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

60min

Standard Curve Gradient

7 Points/3 Folds

Incubation Count

Detectable Sample

Dosing Volume

10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Target

UNIPROT

SwissProt: P39060 Human

Function

Endostatin is a 20 kDa proteolytic fragment of the C-terminal, non-collagenous (NC1) domain of type XVIII Collagen. It was originally identified as a factor produced by murine hemangioendothelioma cells that could specifically inhibit endothelial cell proliferation and angiogenesis. Although the molecular signals that trigger the release of Endostatin from type XVIII Collagen are not well understood, multiple proteases have been suggested to be involved in its generation including Cathepsins S, B, L, and V, Elastase, and matrix metalloproteinases (MMPs)-2, -7, and -9. Endostatin is of particular interest as it has been shown to inhibit the growth of many primary and metastatic tumors. It may also be involved in down-regulating angiogenesis during physiological processes such as wound healing and the establishment of placental circulation. The anti-angiogenic activity of Endostatin is attributable to its ability to inhibit endothelial cell proliferation and suppress VEGF-and FGF basic-induced endothelial cell migration and adhesion. Many of these effects are thought to be mediated by interactions between Endostatin and endothelial cell-expressed Transglutaminase 2, Heparin, and Integrins alpha 5 beta 1 and alpha V beta 3.

Human Endostatin/COL18A1 enzyme-linked immunoassay kit(one step)

Usd: 619 Special Discount

Specification

Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Detectable Sample

Dosing Volume

Product Features

Storage Condition

Target

UNIPROT

Function

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